Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification

Esther Gamero-Sandemetrio; Rocío Gómez-Pastor; Emilia Matallana

doi:10.1007/978-1-4939-7111-4_17

Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification

Esther Gamero-Sandemetrio
, Rocío Gómez-Pastor
, Emilia Matallana

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H₂O₂ allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	189-198
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7111-4_17
State	Published - 2017
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1626
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

Catalase
Electrophoretic isoforms
SOD
Yeast
Zymogram

Publisher link

10.1007/978-1-4939-7111-4_17

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Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification. / Gamero-Sandemetrio, Esther; Gómez-Pastor, Rocío; Matallana, Emilia.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 189-198 (Methods in Molecular Biology; Vol. 1626).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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abstract = "We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.",

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doi = "10.1007/978-1-4939-7111-4\_17",

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AU - Gómez-Pastor, Rocío

AU - Matallana, Emilia

PY - 2017

Y1 - 2017

N2 - We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

AB - We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

KW - Catalase

KW - Electrophoretic isoforms

KW - SOD

KW - Yeast

KW - Zymogram

UR - https://www.scopus.com/pages/publications/85020775097

UR - https://www.scopus.com/pages/publications/85020775097#tab=citedBy

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DO - 10.1007/978-1-4939-7111-4_17

M3 - Chapter

C2 - 28608211

AN - SCOPUS:85020775097

T3 - Methods in Molecular Biology

SP - 189

EP - 198

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification

Abstract

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Keywords

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