TY - JOUR
T1 - Zinc reverse transcriptases from mammalian RNA type C viruses
AU - Auld, D. S.
AU - Kawaguchi, H.
AU - Livingston, D. M.
AU - Vallee, B. L.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1975/1/20
Y1 - 1975/1/20
N2 - 1,10-phenanthroline (OP) reversibly inhibits the RNA dependent DNA polymerases reverse transcriptases from murine, simian, feline and RD-114 RNA tumor viruses. The log of the OP inhibition constants, pKI, vary from 4.7 to 4.9 and the number of moles of inhibitor bound to the metal, ii, vary from 1.3 to 2.2. These values are very similar to those previously reported for known zinc enzymes and imply that the mammalian reverse transcriptases are also zinc enzymes. Combined use of microwave-induced emission spectrometry and micro gel exclusion chromatography demonstrates the presence of stoichiometric amounts of zinc in the enzymes from murine leukemia and woolly monkey type C-viruses. These results parallel those previously reported for the enzyme from avian myeloblastosis virus.
AB - 1,10-phenanthroline (OP) reversibly inhibits the RNA dependent DNA polymerases reverse transcriptases from murine, simian, feline and RD-114 RNA tumor viruses. The log of the OP inhibition constants, pKI, vary from 4.7 to 4.9 and the number of moles of inhibitor bound to the metal, ii, vary from 1.3 to 2.2. These values are very similar to those previously reported for known zinc enzymes and imply that the mammalian reverse transcriptases are also zinc enzymes. Combined use of microwave-induced emission spectrometry and micro gel exclusion chromatography demonstrates the presence of stoichiometric amounts of zinc in the enzymes from murine leukemia and woolly monkey type C-viruses. These results parallel those previously reported for the enzyme from avian myeloblastosis virus.
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U2 - 10.1016/S0006-291X(75)80137-9
DO - 10.1016/S0006-291X(75)80137-9
M3 - Article
C2 - 46146
AN - SCOPUS:0016632783
SN - 0006-291X
VL - 62
SP - 296
EP - 302
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
