Zinc reverse transcriptases from mammalian RNA type C viruses

D. S. Auld, H. Kawaguchi, D. M. Livingston, B. L. Vallee

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


1,10-phenanthroline (OP) reversibly inhibits the RNA dependent DNA polymerases reverse transcriptases from murine, simian, feline and RD-114 RNA tumor viruses. The log of the OP inhibition constants, pKI, vary from 4.7 to 4.9 and the number of moles of inhibitor bound to the metal, ii, vary from 1.3 to 2.2. These values are very similar to those previously reported for known zinc enzymes and imply that the mammalian reverse transcriptases are also zinc enzymes. Combined use of microwave-induced emission spectrometry and micro gel exclusion chromatography demonstrates the presence of stoichiometric amounts of zinc in the enzymes from murine leukemia and woolly monkey type C-viruses. These results parallel those previously reported for the enzyme from avian myeloblastosis virus.

Original languageEnglish (US)
Pages (from-to)296-302
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jan 20 1975

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