ZiFiT (Zinc Finger Targeter) is a simple and intuitive web-based tool that provides an interface to identify potential binding sites for engineered zinc finger proteins (ZFPs) in user-supplied DNA sequences. In this updated version, ZiFiT identifies potential sites for ZFPs made by both the modular assembly and OPEN engineering methods. In addition, ZiFiT now integrates additional tools and resources including scoring schemes for modular assembly, an interface with the Zinc Finger Database (ZiFDB) of engineered ZFPs, and direct querying of NCBI BLAST servers for identifying potential off-target sites within a host genome. Taken together, these features facilitate design of ZFPs using reagents made available to the academic research community by the Zinc Finger Consortium. ZiFiT is freely available on the web without registration at http://bindr.gdcb.iastate.edu/ZiFiT/.
Bibliographical noteFunding Information:
National Institutes of Health [T32CA009216] to J.D.S.; National Science Foundation Graduate Research Fellowship  to M.L.M.; National Science Foundation [DBI 0923827, DBI 0501678] to D.F.V.; National Institutes of Health [R01 GM069906, R01 GM072621, R01 GM088040], the National Science Foundation [DBI 0923827] and Massachusetts General Hospital Pathology Service to J.K.J.; National Science Foundation [DBI 0923827] to D.D. and D.R. Funding for open access charge: National Science Foundation [DBI 0923827].