Workshop Report: Zebrafish and Other Fish Models - Description of Extrinsic Environmental Factors for Rigorous Experiments and Reproducible Results

Zoltan M. Varga, Stephen C. Ekker, Christian Lawrence

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Current standards for husbandry and maintenance of zebrafish and other aquatic species in the laboratory are diverse, and are subject to laboratory performance, engineering, and practice standards (the Guide), institutional interpretation, national animal welfare laws, and cultural differences. Consequently, it is difficult, and probably not advantageous, to establish a single standard in view of the hardy nature of zebrafish and the diversity of research requirements it is used to address. Based on their natural habitat, zebrafish can thrive in a variety of environmental conditions, which is a specific advantage for working with this laboratory organism. However, it also makes reporting and reproducibility difficult, because variations in the husbandry and environmental conditions, including the environmental conditions before and during experiments, are often underreported in the scientific literature. This lack of consistency presents a potential problem for research reproducibility. To begin addressing this emerging scientific gap, the National Institutes of Health's (NIH) Office of Research Infrastructure Programs (ORIP), Division of Construction and Instruments (DCI), hosted a workshop in late 2017, entitled "Zebrafish and Other Aquatic Models: Reporting of Environmental Husbandry Conditions for Rigorous Experiments and Reproducible Results," that was attended by ∼60 participants. The objectives of the workshop were to bring together a diverse group of stakeholders - researchers, facility managers, veterinarians, journal editors, commercial vendors, and others to (1) review current husbandry and environmental management practices for the care of zebrafish and other aquatic organisms in the laboratory and to (2) propose a process for the development of a minimal set of environmental parameters that should be reported in publications to ensure rigor and robustness of experiments and reproducible outcomes. The participants also discussed how these recommendations, as an initial step, might be collected, disseminated, implemented, and improved upon after future iteration.

Original languageEnglish (US)
Pages (from-to)533-535
Number of pages3
Issue number6
StatePublished - Dec 2018

Bibliographical note

Publisher Copyright:
© Copyright 2018, Mary Ann Liebert, Inc., publishers 2018.


  • Danio rerio
  • harmonizaton
  • housing environment
  • husbandry
  • reporting
  • reproducibility
  • scientific rigor


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