Wnt5 signaling in vertebrate pancreas development

Hyon J. Kim, Jack R. Schleiffarth, Jose Jessurun, Saulius Sumanas, Anna Petryk, Shou Lin, Stephen C. Ekker

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Background: Signaling by the Wnt family of secreted glycoproteins through their receptors, the frizzled (Fz) family of seven-pass transmembrane proteins, is critical for numerous cell fate and tissue polarity decisions during development. Results: We report a novel role of Wnt signaling in organogenesis using the formation of the islet during pancreatic development as a model tissue. We used the advantages of the zebrafish to visualize and document this process in living embryos and demonstrated that insulin-positive cells actively migrate to form an islet. We used morpholinos (MOs), sequence-specific translational inhibitors, and time-lapse imaging analysis to show that the Wnt-5 ligand and the Fz-2 receptor are required for proper insulin-cell migration in zebrafish. Histological analyses of islets in Wnt5a-/- mouse embryos showed that Wnt5a signaling is also critical for murine pancreatic insulin-cell migration. Conclusions: Our results implicate a conserved role of a Wnt 5/Fz2 signaling pathway in islet formation during pancreatic development. This study opens the door for further investigation into a role of Wnt signaling in vertebrate organ development and disease.

Original languageEnglish (US)
Article number23
JournalBMC Biology
Volume3
DOIs
StatePublished - Oct 24 2005

Fingerprint Dive into the research topics of 'Wnt5 signaling in vertebrate pancreas development'. Together they form a unique fingerprint.

  • Cite this

    Kim, H. J., Schleiffarth, J. R., Jessurun, J., Sumanas, S., Petryk, A., Lin, S., & Ekker, S. C. (2005). Wnt5 signaling in vertebrate pancreas development. BMC Biology, 3, [23]. https://doi.org/10.1186/1741-7007-3-23