Within the cell: Analytical techniques for subcellular analysis

Karen J. Olson, Hossein Ahmadzadeh, Edgar A. Arriaga

Research output: Contribution to journalReview articlepeer-review

24 Scopus citations

Abstract

This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.

Original languageEnglish (US)
Pages (from-to)906-917
Number of pages12
JournalAnalytical and Bioanalytical Chemistry
Volume382
Issue number4
DOIs
StatePublished - Jun 2005

Bibliographical note

Funding Information:
Acknowledgements Coauthors are supported by NIH grants GM008347 (K.J.O.), R01-AG20866 (H.A.), and 1K02-AG21453 (E.A.A.).

Keywords

  • Electrophoresis
  • Flow cytometry
  • Organelle
  • Subcellular analysis

Fingerprint

Dive into the research topics of 'Within the cell: Analytical techniques for subcellular analysis'. Together they form a unique fingerprint.

Cite this