Within the cell: Analytical techniques for subcellular analysis

Karen J. Olson; Hossein Ahmadzadeh; Edgar A. Arriaga

doi:10.1007/s00216-005-3135-2

Within the cell: Analytical techniques for subcellular analysis

Karen J. Olson, Hossein Ahmadzadeh, Edgar A. Arriaga

Chemistry (Twin Cities)

Research output: Contribution to journal › Review article › peer-review

27 Scopus citations

Abstract

This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.

Original language	English (US)
Pages (from-to)	906-917
Number of pages	12
Journal	Analytical and Bioanalytical Chemistry
Volume	382
Issue number	4
DOIs	https://doi.org/10.1007/s00216-005-3135-2
State	Published - Jun 2005

Bibliographical note

Funding Information:
Acknowledgements Coauthors are supported by NIH grants GM008347 (K.J.O.), R01-AG20866 (H.A.), and 1K02-AG21453 (E.A.A.).

Keywords

Electrophoresis
Flow cytometry
Organelle
Subcellular analysis

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10.1007/s00216-005-3135-2

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title = "Within the cell: Analytical techniques for subcellular analysis",

abstract = "This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.",

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author = "Olson, {Karen J.} and Hossein Ahmadzadeh and Arriaga, {Edgar A.}",

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AU - Ahmadzadeh, Hossein

AU - Arriaga, Edgar A.

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N2 - This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.

AB - This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.

KW - Electrophoresis

KW - Flow cytometry

KW - Organelle

KW - Subcellular analysis

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Within the cell: Analytical techniques for subcellular analysis

Abstract

Bibliographical note

Keywords

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