Within-person variability of the ratios of urinary 2-hydroxyestrone to 16α-hydroxyestrone in Caucasian women

Zhi Chen, Wei Zheng, Lisa M. Dunning, Kristin G. Anderson, Rudolph S. Parrish, Jordan L. Holtzman

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17 Scopus citations

Abstract

The ratio of urinary 2-hydroxyestrone (2-OHE1) to 16α-hydroxyestrone (16α-OHE1) has been suggested as a potential biomarker for breast cancer risk. We evaluated within-person variability of this biomarker in ten healthy Caucasian women aged 23-58 years. Each study participant was asked to provide an overnight fasting morning urine sample once a week for an average of 8 weeks. These urine samples were assayed for 2-OHE1 and 16α-OHE1 by using competitive enzyme immunoassay kits purchased from the ImmunaCare Corporation. The coefficients of variation for urinary 2-OHE1/16α-OHE1 over the study period ranged from 13.7 to 59.6% (mean, 33.3%) in our study participants. There was a good correlation between the level of the urinary 2-OHE1/16α-OHE1 ratio in any single urine sample and the average ratio over the 8-week study period from the same woman, with the mean correlation coefficient of 0.85. These results indicated that the within-person variation of the 2-OHE1 to 16α-OHE1 ratio for most women was moderate and the level of this ratio in a single urine sample, in general, reflects reasonably well the level of this biomarker over a 2-month period.

Original languageEnglish (US)
Pages (from-to)856-859
Number of pages4
JournalSteroids
Volume64
Issue number12
DOIs
StatePublished - Dec 1999

Keywords

  • 2-OHE to 16α-OHE ratio
  • Biomarker
  • Breast cancer
  • Estrogen
  • Estrogen metabolites

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    Chen, Z., Zheng, W., Dunning, L. M., Anderson, K. G., Parrish, R. S., & Holtzman, J. L. (1999). Within-person variability of the ratios of urinary 2-hydroxyestrone to 16α-hydroxyestrone in Caucasian women. Steroids, 64(12), 856-859. https://doi.org/10.1016/S0039-128X(99)00073-2