Wild-type RNA Microhelix(Ala) and 3:70 variants: Molecular dynamics analysis of local helical structure and tightly bound water

Maria C. Nagan, Stephanie S. Kerimo, Karin Musier-Forsyth, Chris Cramer

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39 Scopus citations

Abstract

Molecular dynamics simulations of RNA microhelix(Ala) indicate that G:U and other 3:70 purine: pyrimidine wobble pairs induce local deviations from A-form geometry in their respective microhelices; the helix is underwound at the base-pair step above and overwound at the base-pair step below, in each case by about 7-9°compared to canonical A-form RNA. On the basis of analysis of average water densities and residence lifetimes, the wild-type microhelix strongly binds a water molecule in the minor groove of the 3:70 base pair, consistent with crystallographic analyses of an RNA duplex derived from the acceptor stem of Escherichia coli tRNA(Ala). Other wobble pairs show water binding at this position but to a lesser degree; the strength of water binding correlates directly with the measured aminoacylation activities of the microhelices as substrates for E. coli alanyl-tRNA synthetase (G:U > 2AA:IsoC > G:dU > I:U). Watson-Crick base pairs at the 3:70 position show no tendency toward specific hydration. This tightly bound minor-groove water in the microhelices with 3:70 wobble pairs evidently does not function to stabilize a particular local helical structure, but it may play a role as a specific recognition element or serve as an indicator of interaction specificity between the microhelix and a hydrogen-bonding residue of the aminoacyl-tRNA synthetase.

Original languageEnglish (US)
Pages (from-to)7310-7317
Number of pages8
JournalJournal of the American Chemical Society
Volume121
Issue number32
DOIs
StatePublished - Aug 18 1999

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