Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Shubhada K. Chothe, Aswathy Sebastian, Asha Thomas, Ruth H. Nissly, David Wolfgang, Maurice Byukusenge, Sunil Kumar Mor, Sagar M. Goyal, Istvan Albert, Deepanker Tewari, Bhushan M. Jayarao, Suresh V. Kuchipudi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalVirology
Volume522
DOIs
StatePublished - Sep 2018

Keywords

  • BoHV-1
  • Bovine Herpesvirus-1
  • Complete genomes
  • SNP based PCR assay
  • SNP profiles

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    Chothe, S. K., Sebastian, A., Thomas, A., Nissly, R. H., Wolfgang, D., Byukusenge, M., Mor, S. K., Goyal, S. M., Albert, I., Tewari, D., Jayarao, B. M., & Kuchipudi, S. V. (2018). Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1). Virology, 522, 27-36. https://doi.org/10.1016/j.virol.2018.06.015