Whole-genome characterization and comparative genomics of a novel freshwater cyanobacteria species: Pseudanabaena punensis

Kirti M. Nitnaware, Kiran B. Raskar, Gaurav Agarwal, Ricardo A. Chávez Montes, Ratan Chopra, Damar L. López-Arredondo, Tukaram D. Nikam, Gunvant B. Patil

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Cyanobacteria are emerging as a potential source of novel, beneficial bioactive compounds. However, some cyanobacteria species can harm water quality and public health through the production of toxins. Therefore, surveying the occurrence and generating genomic resources of cyanobacteria producing harmful compounds could help develop the control methods necessary to manage their growth and limit the release contaminants into the water bodies. Here, we describe a novel strain, Pseudanabaena punensis isolated from the open ends of pipelines supplying freshwater. This isolate was characterized morphologically, biochemically and by whole-genome sequence analysis. We also provide genomic information for P. punensis to help understand and highlight the features unique to this isolate. Morphological and genetic (analysis using 16S rRNA and rbcL genes) data were used to assign this novel strain to phylogenetic and taxonomic groups. The isolate was identified as a filamentous and non-heterocystous cyanobacteria. Based on morphological and 16S rRNA phylogeny, this isolate shares characteristics with the Pseudanabaenaceae family, but remains distinct from well-characterized species suggesting its polyphyletic assemblage. The whole-genome sequence analysis suggests greater genomic and phenotypic plasticity. Genome-wide sequence and comparative genomic analyses, comparing against several closely related species, revealed diverse and important genes associated with synthesizing bioactive compounds, multi-drug resistance pathway, heavy metal resistance, and virulence factors. This isolate also produces several important fatty acids with potential industrial applications. The observations described in this study emphasize both industrial applications and risks associated with the freshwater contamination, and therefore genomic resources provided in this study offer an opportunity for further investigations.

Original languageEnglish (US)
Article number107272
JournalMolecular Phylogenetics and Evolution
Volume164
Early online dateJul 28 2021
DOIs
StatePublished - Nov 2021

Bibliographical note

Funding Information:
Author (KMN) is grateful to the Department of Biotechnology (DBT), Government of India, for financial assistance for the project ?Development of image retrieval system for cyanobacteria using foldscope microscope and promotion of use of folds scope for observations in Schools and colleges? BT/IN/Indo-US/Foldscope/39/2015 dated March 20, 2018. GBP and DLL are grateful to the State of Texas' Governor's University Research Initiative (GURI) support and TDN to the UGC, Government of India for UGC-BSR Faculty fellowship. Authors wish to thank Dr. Ahilya Waghmode (Sadguru Gadge Maharaj Collage, Karad, MS), Dr. Abhay Salve (Government Institute of Science, Aurangabad, MS) and Dr. Dr. Manoj Lekhak, (Department of Botany, Shivaji University, Kolhapur, India) for their assistance with classification and microscopic work. This study did not involve humans, human data or animals; no ethics approval or consent is required to publish the results.

Funding Information:
Author (KMN) is grateful to the Department of Biotechnology (DBT), Government of India, for financial assistance for the project “Development of image retrieval system for cyanobacteria using foldscope microscope and promotion of use of folds scope for observations in Schools and colleges” BT/IN/Indo-US/Foldscope/39/2015 dated March 20, 2018. GBP and DLL are grateful to the State of Texas' Governor's University Research Initiative (GURI) support and TDN to the UGC, Government of India for UGC-BSR Faculty fellowship. Authors wish to thank Dr. Ahilya Waghmode (Sadguru Gadge Maharaj Collage, Karad, MS), Dr. Abhay Salve (Government Institute of Science, Aurangabad, MS) and Dr. Dr. Manoj Lekhak, (Department of Botany, Shivaji University, Kolhapur, India) for their assistance with classification and microscopic work.

Publisher Copyright:
© 2021

Keywords

  • Algal bloom
  • Cyanobacteria
  • Drinking water
  • Drug resistance
  • Fatty acid
  • Genome analysis
  • Polyphyletic

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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