Wavy small-diameter vascular graft made of eggshell membrane and thermoplastic polyurethane

Shujie Yan, Brett Napiwocki, Yiyang Xu, Jue Zhang, Xiang Zhang, Xiaofeng Wang, Wendy C. Crone, Qian Li, Lih Sheng Turng

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


In this study, a small-diameter, double-layered eggshell membrane/thermoplastic polyurethane (ESM/TPU) vascular graft with a wavy structure was developed. The avian eggshell membrane, a fibrous structure similar to the extracellular matrix (ECM), has the potential to yield rapid endothelialization in vitro. The dopamine and heparin modification of the ESM surface not only promoted human umbilical vein endothelial cell (HUVEC) proliferation via cytocompatibility assessment, but also improved its anticoagulation properties as verified in platelet adhesion tests. The biomimetic mechanical properties of the vascular graft were provided by the elastic TPU fibers via electrospinning using a wavy cross-section rotating collector. The advantage of combining these two materials is to make use of the bioactivity of ESM as the internal membrane and the tunable mechanical properties of TPU as the external layer. The circumferentially wavy structure of the vascular graft produced a toe region in the non-linear section of the stress–strain curve similar to that of natural blood vessels. The ESM/TPU graft's circumferential ultimate strength was 2.57 MPa, its strain was 339% mm/mm, and its toe region was found to be around 20% mm/mm. Cyclical tension tests showed that the vascular graft could maintain good mechanical properties and showed no structural damage under repeated extension tests.

Original languageEnglish (US)
Article number110311
JournalMaterials Science and Engineering C
StatePublished - Feb 2020
Externally publishedYes

Bibliographical note

Funding Information:
This research reported was partially supported by the NHLBI of the National Institutes of Health under award number U01HL134655 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health . Support was also provided by the Kuo K. and Cindy F. Wang Professorship and the Office of the Vice Chancellor for Research and Graduate Education at the University of Wisconsin–Madison. The authors would like to thank the Wisconsin Institute for Discovery at the University of Wisconsin–Madison for the experimental lab and equipment, the National Center for International Research of Micro-Nano Molding Technology at Zhengzhou University, the International Science & Technology Cooperation Program of China (No. 2015DFA30550 ), and the China Scholarship Council for their financial support ( 201707040014 ).

Publisher Copyright:
© 2019 Elsevier B.V.

Copyright 2019 Elsevier B.V., All rights reserved.


  • Antithrombogenicity
  • Eggshell membrane
  • Endothelialization
  • Small-diameter vascular graft
  • Thermoplastic polyurethane

PubMed: MeSH publication types

  • Journal Article


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