"water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation

Hua Zhao; Glenn A. Harter; Caden J. Martin

doi:10.1021/acsomega.9b02118

"water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation

Hua Zhao, Glenn A. Harter, Caden J. Martin

Research output: Contribution to journal › Article › peer-review

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Abstract

By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH₃(OCH₂CH₂)_n-Im-t-BuOH][Tf₂N]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2-4 folds higher than nonfunctionalized "classical" ionic liquids (such as [BMIM][Tf₂N]) and up to 40-100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol.

Original language	English (US)
Pages (from-to)	15234-15239
Number of pages	6
Journal	ACS Omega
Volume	4
Issue number	12
DOIs	https://doi.org/10.1021/acsomega.9b02118
State	Published - Sep 17 2019
Externally published	Yes

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Copyright © 2019 American Chemical Society.

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abstract = "By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH3(OCH2CH2)n-Im-t-BuOH][Tf2N]). This unique combination of the {"}water-like{"} structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2-4 folds higher than nonfunctionalized {"}classical{"} ionic liquids (such as [BMIM][Tf2N]) and up to 40-100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol.",

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T1 - "water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation

AU - Zhao, Hua

AU - Harter, Glenn A.

AU - Martin, Caden J.

PY - 2019/9/17

Y1 - 2019/9/17

N2 - By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH3(OCH2CH2)n-Im-t-BuOH][Tf2N]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2-4 folds higher than nonfunctionalized "classical" ionic liquids (such as [BMIM][Tf2N]) and up to 40-100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol.

AB - By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH3(OCH2CH2)n-Im-t-BuOH][Tf2N]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2-4 folds higher than nonfunctionalized "classical" ionic liquids (such as [BMIM][Tf2N]) and up to 40-100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol.

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"water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation

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