Wakefulness/sleep architecture and electroencephalographic activity in mice lacking the translational repressor 4E-BP1 or 4E-BP2

Cassandra C. Areal, Ruifeng Cao, Nahum Sonenberg, Valérie Mongrain

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4 Scopus citations


Sleep and sleep loss are affecting protein synthesis in the brain, but the contribution of translational control to wakefulness and sleep regulation remains poorly understood. Here, we studied the role of two suppressors of protein synthesis, the eukaryotic translation initiation factor 4E-binding proteins 1 and 2 (4E-BP1 and 4E-BP2), in sleep architecture and electroencephalographic (EEG) activity as well as in the EEG and molecular responses to acute sleep loss. The EEG of mice mutant for the genes encoding 4E-BP1 or 4E-BP2 (Eif4ebp1 and Eif4ebp2 knockout [KO] mice) was recorded under undisturbed conditions and following a 6-hour sleep deprivation (SD). The effect of SD on the expression of genes known to respond to SD was also measured in the prefrontal cortex of Eif4ebp1 and Eif4ebp2 KO mice. Eif4ebp1 KO mice differed from wild-type mice in parameters of wakefulness and sleep quantity and quality, and more subtly in the gene expression response to SD. For instance, Eif4ebp1 KO mice spent more time in slow-wave sleep (SWS) and showed altered baseline 24-h time courses of SWS delta (1-4 Hz) activity and sigma (10-13 Hz) activity. Eif4ebp2 KO mice differed from wild-type mice only for wakefulness and sleep quality, expressing changes in EEG spectral activity generally revealed during and after SD. These findings suggest different roles of effectors of translational control in the regulation of wakefulness and sleep and of synchronized cortical activity.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
Issue number2
StatePublished - Feb 13 2020

Bibliographical note

Funding Information:
The research has been supported by M.Sc. fellowships of the Department of Neuroscience and of the Faculty of Medicine of the Université de Montréal to C.C.A., salary awards from the Canadian Institutes of Health Research (CIHR) and the Fonds de Recherche du Québec-Santé to V.M., the Canada Research Chair in Sleep Molecular Physiology (V.M.), and a CIHR grant to N.S. Conflict of interest statement. None declared.

Publisher Copyright:
© 2019 Sleep Research Society 2019. Published by Oxford University Press on behalf of the Sleep Research Society. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.


  • electroencephalography
  • gene expression
  • protein synthesis
  • sleep deprivation
  • spectral analysis

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't


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