VS38 Identifies Myeloma Cells with Dim CD38 Expression and Plasma Cells Following Daratumumab Therapy, Which Interferes with CD38 Detection for 4 to 6 Months

Elizabeth L. Courville, Sophia Yohe, Paula Shivers, Michael A. Linden

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Objectives: We report our institutional experience using VS38 to evaluate plasma cells by flow cytometry. Methods: Flow cytometry data were reanalyzed to compare plasma cell percentages between the standard panel and VS38 panel. Natural killer (NK) and plasma cell CD38 median fluorescence intensity (MFI) values were calculated. Results: Our cohort included 63 specimens from 38 patients. Twenty-six had received daratumumab (monoclonal anti-CD38 therapy) between less than 1 month and 17 months prior. For NK and plasma cells, CD38 MFI values were suppressed for 0 to 4 months and started to increase 4 to 6 months after last exposure. There was no significant difference in clonal plasma cell percentage calculated by the VS38 and standard panels; however, identification and quantification using the VS38 panel were easier. Conclusions: VS38 is a viable alternative to bright CD38 to identify plasma cells and particularly helpful in myeloma cases with dim CD38 and after daratumumab. Daratumumab interference with CD38 identification persists 4 to 6 months after the last exposure.

Original languageEnglish (US)
Pages (from-to)221-228
Number of pages8
JournalAmerican journal of clinical pathology
Volume153
Issue number2
DOIs
StatePublished - Jan 2 2020

Keywords

  • CD38
  • Daratumumab
  • Flow cytometric immunophenotyping
  • Flow cytometry
  • Monoclonal antibody therapy
  • Multiple myeloma
  • Plasma cell myeloma
  • Plasma cell neoplasm
  • Plasma cells
  • VS38

PubMed: MeSH publication types

  • Journal Article

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