VP6 genetic diversity, reassortment, intragenic recombination and classification of rotavirus B in American and Japanese pigs

Douglas Marthaler; Tohru Suzuki; Kurt Rossow; Marie Culhane; James Collins; Sagar Goyal; Hiroshi Tsunemitsu; Max Ciarlet; Jelle Matthijnssens

doi:10.1016/j.vetmic.2014.05.015

VP6 genetic diversity, reassortment, intragenic recombination and classification of rotavirus B in American and Japanese pigs

Douglas Marthaler, Tohru Suzuki, Kurt Rossow, Marie Culhane, James Collins, Sagar Goyal, Hiroshi Tsunemitsu, Max Ciarlet, Jelle Matthijnssens

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Rotavirus B (RVB) has been identified as a causative agent of diarrhea in rats, humans, cattle, lambs, and swine. Recently, 20 RVB VP7 genotypes were determined based on an 80% nucleotide percent cut-off value. In this study, we sequenced the RVB VP6 gene segment from 80 RVB positive swine samples from the United States and Japan. Phylogenetic analyses, using the 30 available RVB VP6 sequences from GenBank and our 80 novel RVB VP6 sequences, revealed a large genetic diversity of RVB strains, mainly in pigs. For classification purposes, pairwise identity frequency analyses suggested an 81% nucleotide percent cut-off value, resulting in 13 RVB VP6 (I) genotypes. In addition, an intragenic recombinant RVB VP6 segment was identified from Japan. Furthermore, the data indicates frequent reassortment events occurred between the porcine RVB VP7 and VP6 gene segments.

Original language	English (US)
Pages (from-to)	359-366
Number of pages	8
Journal	Veterinary Microbiology
Volume	172
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2014.05.015
State	Published - Aug 27 2014

Bibliographical note

Funding Information:
The Japanese RVB sequencing was funded in part by a grant-in-aid for scientific research from the Ministry of Health, Labor and Welfare of Japan and by a grant from the National Institute of Animal Health, National Agriculture and Food Research Organization , Japan. The USA RVB sequencing and phylogenetic analysis was funded by the University of Minnesota Veterinary Diagnostic Laboratory .

Keywords

Classification
Genetic diversity
Intragenic recombination
Porcine enteric pathogen
Reassortment
Rotavirus B

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetmic.2014.05.015

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title = "VP6 genetic diversity, reassortment, intragenic recombination and classification of rotavirus B in American and Japanese pigs",

abstract = "Rotavirus B (RVB) has been identified as a causative agent of diarrhea in rats, humans, cattle, lambs, and swine. Recently, 20 RVB VP7 genotypes were determined based on an 80% nucleotide percent cut-off value. In this study, we sequenced the RVB VP6 gene segment from 80 RVB positive swine samples from the United States and Japan. Phylogenetic analyses, using the 30 available RVB VP6 sequences from GenBank and our 80 novel RVB VP6 sequences, revealed a large genetic diversity of RVB strains, mainly in pigs. For classification purposes, pairwise identity frequency analyses suggested an 81% nucleotide percent cut-off value, resulting in 13 RVB VP6 (I) genotypes. In addition, an intragenic recombinant RVB VP6 segment was identified from Japan. Furthermore, the data indicates frequent reassortment events occurred between the porcine RVB VP7 and VP6 gene segments.",

keywords = "Classification, Genetic diversity, Intragenic recombination, Porcine enteric pathogen, Reassortment, Rotavirus B",

author = "Douglas Marthaler and Tohru Suzuki and Kurt Rossow and Marie Culhane and James Collins and Sagar Goyal and Hiroshi Tsunemitsu and Max Ciarlet and Jelle Matthijnssens",

note = "Funding Information: The Japanese RVB sequencing was funded in part by a grant-in-aid for scientific research from the Ministry of Health, Labor and Welfare of Japan and by a grant from the National Institute of Animal Health, National Agriculture and Food Research Organization , Japan. The USA RVB sequencing and phylogenetic analysis was funded by the University of Minnesota Veterinary Diagnostic Laboratory . ",

year = "2014",

month = aug,

day = "27",

doi = "10.1016/j.vetmic.2014.05.015",

language = "English (US)",

volume = "172",

pages = "359--366",

journal = "Veterinary Microbiology",

issn = "0378-1135",

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T1 - VP6 genetic diversity, reassortment, intragenic recombination and classification of rotavirus B in American and Japanese pigs

AU - Marthaler, Douglas

AU - Suzuki, Tohru

AU - Rossow, Kurt

AU - Culhane, Marie

AU - Collins, James

AU - Goyal, Sagar

AU - Tsunemitsu, Hiroshi

AU - Ciarlet, Max

AU - Matthijnssens, Jelle

N1 - Funding Information: The Japanese RVB sequencing was funded in part by a grant-in-aid for scientific research from the Ministry of Health, Labor and Welfare of Japan and by a grant from the National Institute of Animal Health, National Agriculture and Food Research Organization , Japan. The USA RVB sequencing and phylogenetic analysis was funded by the University of Minnesota Veterinary Diagnostic Laboratory .

PY - 2014/8/27

Y1 - 2014/8/27

N2 - Rotavirus B (RVB) has been identified as a causative agent of diarrhea in rats, humans, cattle, lambs, and swine. Recently, 20 RVB VP7 genotypes were determined based on an 80% nucleotide percent cut-off value. In this study, we sequenced the RVB VP6 gene segment from 80 RVB positive swine samples from the United States and Japan. Phylogenetic analyses, using the 30 available RVB VP6 sequences from GenBank and our 80 novel RVB VP6 sequences, revealed a large genetic diversity of RVB strains, mainly in pigs. For classification purposes, pairwise identity frequency analyses suggested an 81% nucleotide percent cut-off value, resulting in 13 RVB VP6 (I) genotypes. In addition, an intragenic recombinant RVB VP6 segment was identified from Japan. Furthermore, the data indicates frequent reassortment events occurred between the porcine RVB VP7 and VP6 gene segments.

AB - Rotavirus B (RVB) has been identified as a causative agent of diarrhea in rats, humans, cattle, lambs, and swine. Recently, 20 RVB VP7 genotypes were determined based on an 80% nucleotide percent cut-off value. In this study, we sequenced the RVB VP6 gene segment from 80 RVB positive swine samples from the United States and Japan. Phylogenetic analyses, using the 30 available RVB VP6 sequences from GenBank and our 80 novel RVB VP6 sequences, revealed a large genetic diversity of RVB strains, mainly in pigs. For classification purposes, pairwise identity frequency analyses suggested an 81% nucleotide percent cut-off value, resulting in 13 RVB VP6 (I) genotypes. In addition, an intragenic recombinant RVB VP6 segment was identified from Japan. Furthermore, the data indicates frequent reassortment events occurred between the porcine RVB VP7 and VP6 gene segments.

KW - Classification

KW - Genetic diversity

KW - Intragenic recombination

KW - Porcine enteric pathogen

KW - Reassortment

KW - Rotavirus B

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VP6 genetic diversity, reassortment, intragenic recombination and classification of rotavirus B in American and Japanese pigs

Abstract

Bibliographical note

Keywords

UN SDGs

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