Voltage-dependent inhibition of Ca2+ channels in GH3 cells by cloned μ- and δ-opioid receptors

Elemer T. Piros, Paul L. Prather, Ping Y. Law, Chris J. Evans, Tim G. Hales

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

To study cloned opioid receptor binding and modulation of both adenylyl cyclase and ion channel activity, we stably expressed μ- and δ-opioid receptors in the rodent pituitary-derived GH3 cell line. GH3 cells express G proteins and voltage-activated Ca2+ channels (predominantly of the L- type). Activation of cloned rat μ-opioid receptors expressed in GH3 cells (termed GH3MOR cells) inhibits L-type Ca2+ channel activity. GH3MOR cells, further transfected with mouse δ receptor cDNA (termed GH3MORDOR cells), bound both [D-Ala2,N-MePhe4,Gly-ol5]enkephalin (DAMGO) and [D- Pen2,D-Pen5]enkephalin (DPDPE). These opioid ligands inhibited adenylyl cyclase activity (IC50 = 174 and 0.53 nM, respectively). This action of DAMGO and DPDPE was attenuated selectively by μ- and δ-opioid receptor- specific antagonists. Activation of both opioid receptors also led to inhibition of Ca2+ channel activity, measured with Ba2+ as the charge carrier using the whole-cell patch-clamp technique. Both DAMGO (1 μM) and DPDPE (1 μM) reversibly inhibited Ba2+ currents (by 17.0 ± 1.4% and 20.7 ± 1.3%, respectively) in GH3MORDOR cells. The inhibitory action of DPDPE was close dependent (IC50 = 1.6 nM) and was attenuated by pretreatment with pertussis toxin (200 ng/ml) or by the inclusion of guanosine-5'-O-(2- thio)diphosphate (2 mM) in the recording electrode. Ba2+ current inhibitions by both DAMGO and DPDPE were completely reversed by depolarizing (to >50 mV) prepulses in GH3MORDOR cells. In summary, cloned μ- and δ- opioid receptors expressed in GH3 cells voltage-dependently couple through G(i)G(o) proteins to L-type Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)947-956
Number of pages10
JournalMolecular Pharmacology
Volume50
Issue number4
StatePublished - Oct 1996

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    Piros, E. T., Prather, P. L., Law, P. Y., Evans, C. J., & Hales, T. G. (1996). Voltage-dependent inhibition of Ca2+ channels in GH3 cells by cloned μ- and δ-opioid receptors. Molecular Pharmacology, 50(4), 947-956.