TY - JOUR
T1 - Voltage-dependent inhibition of Ca2+ channels in GH3 cells by cloned μ- and δ-opioid receptors
AU - Piros, Elemer T.
AU - Prather, Paul L.
AU - Law, Ping Y.
AU - Evans, Chris J.
AU - Hales, Tim G.
PY - 1996/10
Y1 - 1996/10
N2 - To study cloned opioid receptor binding and modulation of both adenylyl cyclase and ion channel activity, we stably expressed μ- and δ-opioid receptors in the rodent pituitary-derived GH3 cell line. GH3 cells express G proteins and voltage-activated Ca2+ channels (predominantly of the L- type). Activation of cloned rat μ-opioid receptors expressed in GH3 cells (termed GH3MOR cells) inhibits L-type Ca2+ channel activity. GH3MOR cells, further transfected with mouse δ receptor cDNA (termed GH3MORDOR cells), bound both [D-Ala2,N-MePhe4,Gly-ol5]enkephalin (DAMGO) and [D- Pen2,D-Pen5]enkephalin (DPDPE). These opioid ligands inhibited adenylyl cyclase activity (IC50 = 174 and 0.53 nM, respectively). This action of DAMGO and DPDPE was attenuated selectively by μ- and δ-opioid receptor- specific antagonists. Activation of both opioid receptors also led to inhibition of Ca2+ channel activity, measured with Ba2+ as the charge carrier using the whole-cell patch-clamp technique. Both DAMGO (1 μM) and DPDPE (1 μM) reversibly inhibited Ba2+ currents (by 17.0 ± 1.4% and 20.7 ± 1.3%, respectively) in GH3MORDOR cells. The inhibitory action of DPDPE was close dependent (IC50 = 1.6 nM) and was attenuated by pretreatment with pertussis toxin (200 ng/ml) or by the inclusion of guanosine-5'-O-(2- thio)diphosphate (2 mM) in the recording electrode. Ba2+ current inhibitions by both DAMGO and DPDPE were completely reversed by depolarizing (to >50 mV) prepulses in GH3MORDOR cells. In summary, cloned μ- and δ- opioid receptors expressed in GH3 cells voltage-dependently couple through G(i)G(o) proteins to L-type Ca2+ channels.
AB - To study cloned opioid receptor binding and modulation of both adenylyl cyclase and ion channel activity, we stably expressed μ- and δ-opioid receptors in the rodent pituitary-derived GH3 cell line. GH3 cells express G proteins and voltage-activated Ca2+ channels (predominantly of the L- type). Activation of cloned rat μ-opioid receptors expressed in GH3 cells (termed GH3MOR cells) inhibits L-type Ca2+ channel activity. GH3MOR cells, further transfected with mouse δ receptor cDNA (termed GH3MORDOR cells), bound both [D-Ala2,N-MePhe4,Gly-ol5]enkephalin (DAMGO) and [D- Pen2,D-Pen5]enkephalin (DPDPE). These opioid ligands inhibited adenylyl cyclase activity (IC50 = 174 and 0.53 nM, respectively). This action of DAMGO and DPDPE was attenuated selectively by μ- and δ-opioid receptor- specific antagonists. Activation of both opioid receptors also led to inhibition of Ca2+ channel activity, measured with Ba2+ as the charge carrier using the whole-cell patch-clamp technique. Both DAMGO (1 μM) and DPDPE (1 μM) reversibly inhibited Ba2+ currents (by 17.0 ± 1.4% and 20.7 ± 1.3%, respectively) in GH3MORDOR cells. The inhibitory action of DPDPE was close dependent (IC50 = 1.6 nM) and was attenuated by pretreatment with pertussis toxin (200 ng/ml) or by the inclusion of guanosine-5'-O-(2- thio)diphosphate (2 mM) in the recording electrode. Ba2+ current inhibitions by both DAMGO and DPDPE were completely reversed by depolarizing (to >50 mV) prepulses in GH3MORDOR cells. In summary, cloned μ- and δ- opioid receptors expressed in GH3 cells voltage-dependently couple through G(i)G(o) proteins to L-type Ca2+ channels.
UR - http://www.scopus.com/inward/record.url?scp=0029859922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029859922&partnerID=8YFLogxK
M3 - Article
C2 - 8863841
AN - SCOPUS:0029859922
SN - 0026-895X
VL - 50
SP - 947
EP - 956
JO - Molecular Pharmacology
JF - Molecular Pharmacology
IS - 4
ER -