Vitamin e in macular and peripheral tissues of the human Eye

Tim Friedrichson, Harrison Levan Kalbach, Paul Buck, Frederik J.G.M. van Kuijk

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

This study was undertaken to investigate the distribution of vitamin E in the macular and peripheral regions of the human retina, retinal pigment epithelium (RPE) and choroid as a function of age. High-performance liquid chromatography (HPLC) was used to measure α and γtocopherol levels quantitatively using tocol as an internal standard. In 57 out of 70 donor eyes (ages 9-104) the macular region was isolated and the tocopherols analyzed. The conventional brush method and a new vortex method were used to isolate the retinal pigment epithelium cells. Similar trends for the vitamin E levels (increase to the 5th decade, decrease after 7th decade) were found for the macular and peripheral retina and for the macular RPE. In the peripheral RPE a slight continuous increase with age was found. The vitamin E levels are higher in the RPE than in the retina, for both macular and peripheral regions. The amounts of vitamin E/mg protein are lower in the macular retina than in the peripheral retina, whereas in the RPE there is no difference in vitamin E content between macular and peripheral regions. A simple method based on a gentle vortex step was found to offer several advantages over the more generally used isolation of RPE cells based on brushing, and there was no difference in recovery of vitamin E in RPE cells when they were isolated by either isolation technique. It was also found that denominators, used to express the values of vitamin E in tissues should, be used with care since age dependent trends in parameters/denominators could be caused by trends in the denominators only.

Original languageEnglish (US)
Pages (from-to)693-701
Number of pages9
JournalCurrent Eye Research
Volume14
Issue number8
DOIs
StatePublished - Jan 1 1995

Keywords

  • Age-related macular degeneration (ARMD)
  • Antioxidants
  • Human
  • Retina
  • Retinal pigment epithelium (RPE)
  • Vitamin E

Fingerprint Dive into the research topics of 'Vitamin e in macular and peripheral tissues of the human Eye'. Together they form a unique fingerprint.

Cite this