Vital staining of corneal endothelium

G. Singh, M. Bohnke, D. Von-Domarus, J. Draeger, R. L. Lindstrom, D. J. Doughman

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


The efficacy and applicability of dual vital staining of human and porcine corneal endothelium with trypan blue and alizarin red S stains was investigated. Exposure of endothelial cells to different concentrations of these two dyes for varying periods of time revealed that 0.3% solution of trypan blue for 1 minute followed by 0.2% solution of alizarin red S (pH 4.2) for 1/2 to 1 minute was the most useful technique to assess the cell viability, micro to macro damage, cell division and cell counts, etc. Alizarin red S dye should not be used in in vivo evaluation, while trypan blue was very effective to evaluate the endothelial viability of donor cornea just prior to keratoplasty.

Original languageEnglish (US)
Pages (from-to)80-91
Number of pages12
Issue number2
StatePublished - Dec 1 1985


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