Visualizing mrna dynamics in live neurons  and brain tissues

Hye Yoon Park; Minho Song

doi:10.1007/978-1-4939-3067-8_21

Visualizing mrna dynamics in live neurons and brain tissues

Hye Yoon Park
, Minho Song

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Localization of mRNA plays a crucial role in a variety of neuronal processes including synaptogenesis, axonal guidance, and long-term plasticity. Recent advances in fl uorescence imaging and RNA labeling techniques allow us to visualize how individual mRNA molecules are dynamically regulated inside live neurons and brain tissues. Here, we describe key methods in imaging mRNA dynamics, including preparation of neuron culture and brain slices from transgenic mice expressing GFP-labeled mRNA, highresolution detection of single molecules, live tissue imaging, and analysis of mRNA transport.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	325-334
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-3067-8_21
State	Published - 2016
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1358
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
©Springer Science+Business Media New York 2016

Keywords

Live cell imaging
MS2-GFP system
RNA localization
Single-molecule imaging
Twophoton microscopy

Publisher link

10.1007/978-1-4939-3067-8_21

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KW - MS2-GFP system

KW - RNA localization

KW - Single-molecule imaging

KW - Twophoton microscopy

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