Visualizing and mapping the cerebellum with serial optical coherence scanner

Chao J. Liu; Kristen E. Williams; Harry T. Orr; Taner Akkin

doi:10.1117/1.NPh.4.1.011006

Visualizing and mapping the cerebellum with serial optical coherence scanner

Chao J. Liu, Kristen E. Williams, Harry T. Orr, Taner Akkin

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.

Original language	English (US)
Article number	011006
Journal	Neurophotonics
Volume	4
Issue number	1
DOIs	https://doi.org/10.1117/1.NPh.4.1.011006
State	Published - Jan 1 2017

Bibliographical note

Funding Information:
Acknowledgments This work was supported by research grants from the US National Science Foundation (No. NSF, CBET-1510674) and the Bob Allison Ataxia Research Center (BAARC) at the University of Minnesota. The authors would like to thank Orion Rainwater for helping with tissue preparation, and Minnesota Supercomputing Institute for the high-performance computing resources.

Keywords

Cerebellum
brainstem
fiber orientation
optical coherence tomography
polarization

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abstract = "We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.",

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