We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.
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Acknowledgments This work was supported by research grants from the US National Science Foundation (No. NSF, CBET-1510674) and the Bob Allison Ataxia Research Center (BAARC) at the University of Minnesota. The authors would like to thank Orion Rainwater for helping with tissue preparation, and Minnesota Supercomputing Institute for the high-performance computing resources.
- fiber orientation
- optical coherence tomography