Visualizing and mapping the cerebellum with serial optical coherence scanner

Chao J. Liu, Kristen E. Williams, Harry T. Orr, Taner Akkin

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.

Original languageEnglish (US)
Article number011006
JournalNeurophotonics
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2017

Bibliographical note

Publisher Copyright:
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).

Keywords

  • Cerebellum
  • brainstem
  • fiber orientation
  • optical coherence tomography
  • polarization

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