Abstract
We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.
| Original language | English (US) |
|---|---|
| Article number | 011006 |
| Journal | Neurophotonics |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2017 |
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Keywords
- Cerebellum
- brainstem
- fiber orientation
- optical coherence tomography
- polarization
Cite this
Visualizing and mapping the cerebellum with serial optical coherence scanner. / Liu, Chao J.; Williams, Kristen E.; Orr, Harry T; Akkin, Taner.
In: Neurophotonics, Vol. 4, No. 1, 011006, 01.01.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Visualizing and mapping the cerebellum with serial optical coherence scanner
AU - Liu, Chao J.
AU - Williams, Kristen E.
AU - Orr, Harry T
AU - Akkin, Taner
PY - 2017/1/1
Y1 - 2017/1/1
N2 - We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.
AB - We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.
KW - Cerebellum
KW - brainstem
KW - fiber orientation
KW - optical coherence tomography
KW - polarization
UR - http://www.scopus.com/inward/record.url?scp=84990053686&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990053686&partnerID=8YFLogxK
U2 - 10.1117/1.NPh.4.1.011006
DO - 10.1117/1.NPh.4.1.011006
M3 - Article
VL - 4
JO - Neurophotonics
JF - Neurophotonics
SN - 2329-4248
IS - 1
M1 - 011006
ER -