Visualization of the genesis and fate of isotype-switched B cells during a primary immune response

Kathryn A Pape, Valerie Kouskoff, David Nemazee, H. Lucy Tang, Jason G. Cyster, Lina E. Tze, Keli L Hippen, Timothy W. Behrens, Marc Jenkins

Research output: Contribution to journalArticlepeer-review

111 Scopus citations

Abstract

The life history of isotype-switched B cells is unclear, in part, because of an inability to detect rare antigen-specific B cells at early times during the immune response. To address this issue, a small population of B cells carrying targeted antibody transgenes capable of class switching was monitored in immunized mice. After contacting helper T cells, the first switched B cells appeared in follicles rather than in the red pulp, as was expected. Later, some of the switched B cells transiently occupied the red pulp and marginal zone, whereas others persisted in germinal centers (GCs). Antigen-experienced IgM B cells were rarely found in GCs, indicating that these cells switched rapidly after entering GCs or did not persist in this environment.

Original languageEnglish (US)
Pages (from-to)1677-1687
Number of pages11
JournalJournal of Experimental Medicine
Volume197
Issue number12
DOIs
StatePublished - Jun 16 2003

Keywords

  • Germinal centers
  • Lymphocyte activation
  • Marginal zone
  • T cells
  • Transgenic mice

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