Understanding the complex architecture, connectivity, and pathology of the human brain is a major application of magnetic resonance imaging (MRI). However, the cellular basis of MR signal is still poorly understood. The advent of MR microscopy (MRM) enables imaging biological samples at cellular resolution, helping to interpret the nature of MR signal at the cellular level. In this regard, the small Drosophila brain can reveal key aspects of MR signal through the visualization of complex, intact neuronal structures in their native spatial arrangement. Applying state-of-the-art MR technology, we imaged fixed Drosophila heads at 10â €...Î 1/4m isotropic resolution by two endogenously contrasted MRM sequences. The improved MRM sensitivity described here delivered the highest 3D resolution of an intact animal head reported so far. 3D fast low angle shot (FLASH) revealed strong signal in most internal tissues, particularly in the brain cortex, which contains the cell bodies of neurons and glia. Remarkably, 3D diffusion weighted imaging (DWI) delivered unprecedented contrast within the modular brain neuropil, revealing hyperintense signal in synapse-rich microdomains. Thus, the complex Drosophila brain revealed unknown features of FLASH and DWI with potential applications in characterizing the structure and pathology of the mammalian brain.
|Original language||English (US)|
|State||Published - Mar 2015|
Bibliographical noteFunding Information:
We would like to thank members of the AMRIS staff at the University of Florida’s McKnight Brain Institute for technical support. This work was supported the NIH grant 1R01EB012874-01 to SJB, and a McKnight Brain Institute Research Development Award (UF Project 00112640), start-up funds from the UF department of Neurology, and a small user award (P07157) from the National High Magnetic Field Laboratory to PFF.