Visualization of dynamics of single endogenous mRNA labeled in live mouse

Hye Yoon Park; Hyungsik Lim; Young J. Yoon; Antonia Follenzi; Chiso Nwokafor; Melissa Lopez-Jones; Xiuhua Meng; Robert H. Singer

doi:10.1126/science.1239200

Visualization of dynamics of single endogenous mRNA labeled in live mouse

Hye Yoon Park, Hyungsik Lim, Young J. Yoon, Antonia Follenzi, Chiso Nwokafor, Melissa Lopez-Jones, Xiuhua Meng, Robert H. Singer

Research output: Contribution to journal › Article › peer-review

222 Scopus citations

Abstract

Thetranscription and transport of messenger RNA (mRNA) are critical steps in regulating the spatial and temporal components of gene expression, butit has not been possible to observe the dynamics of endogenous mRNA in primary mammalian tissues. We have developed a transgenic mouse in which all -βactin mRNA is fluorescently labeled. We found that β-actin mRNA in primary fibroblasts localizes predominantly by diffusion and trapping as single mRNAs. In cultured neurons and acute brain slices, wefound that multiple -βactin mRNAs can assemble together, travel by active transport, anddisassemble upon depolarization by potassium chloride. Imaging of brain slices revealed immediate early induction of -βactin transcription after depolarization. Studying endogenous mRNA in live mouse tissues provides insight into its dynamic regulation within the context of the cellular and tissue microenvironment.

Original language	English (US)
Pages (from-to)	422-424
Number of pages	3
Journal	Science
Volume	343
Issue number	6169
DOIs	https://doi.org/10.1126/science.1239200
State	Published - 2014
Externally published	Yes

Publisher link

10.1126/science.1239200

Find It

Find It at U of M Libraries

Cite this

@article{83f6a609c0b0443b92aefbe8ab3bd0ee,

title = "Visualization of dynamics of single endogenous mRNA labeled in live mouse",

abstract = "Thetranscription and transport of messenger RNA (mRNA) are critical steps in regulating the spatial and temporal components of gene expression, butit has not been possible to observe the dynamics of endogenous mRNA in primary mammalian tissues. We have developed a transgenic mouse in which all -βactin mRNA is fluorescently labeled. We found that β-actin mRNA in primary fibroblasts localizes predominantly by diffusion and trapping as single mRNAs. In cultured neurons and acute brain slices, wefound that multiple -βactin mRNAs can assemble together, travel by active transport, anddisassemble upon depolarization by potassium chloride. Imaging of brain slices revealed immediate early induction of -βactin transcription after depolarization. Studying endogenous mRNA in live mouse tissues provides insight into its dynamic regulation within the context of the cellular and tissue microenvironment.",

author = "Park, {Hye Yoon} and Hyungsik Lim and Yoon, {Young J.} and Antonia Follenzi and Chiso Nwokafor and Melissa Lopez-Jones and Xiuhua Meng and Singer, {Robert H.}",

year = "2014",

doi = "10.1126/science.1239200",

language = "English (US)",

volume = "343",

pages = "422--424",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6169",

}

TY - JOUR

T1 - Visualization of dynamics of single endogenous mRNA labeled in live mouse

AU - Park, Hye Yoon

AU - Lim, Hyungsik

AU - Yoon, Young J.

AU - Follenzi, Antonia

AU - Nwokafor, Chiso

AU - Lopez-Jones, Melissa

AU - Meng, Xiuhua

AU - Singer, Robert H.

PY - 2014

Y1 - 2014

N2 - Thetranscription and transport of messenger RNA (mRNA) are critical steps in regulating the spatial and temporal components of gene expression, butit has not been possible to observe the dynamics of endogenous mRNA in primary mammalian tissues. We have developed a transgenic mouse in which all -βactin mRNA is fluorescently labeled. We found that β-actin mRNA in primary fibroblasts localizes predominantly by diffusion and trapping as single mRNAs. In cultured neurons and acute brain slices, wefound that multiple -βactin mRNAs can assemble together, travel by active transport, anddisassemble upon depolarization by potassium chloride. Imaging of brain slices revealed immediate early induction of -βactin transcription after depolarization. Studying endogenous mRNA in live mouse tissues provides insight into its dynamic regulation within the context of the cellular and tissue microenvironment.

AB - Thetranscription and transport of messenger RNA (mRNA) are critical steps in regulating the spatial and temporal components of gene expression, butit has not been possible to observe the dynamics of endogenous mRNA in primary mammalian tissues. We have developed a transgenic mouse in which all -βactin mRNA is fluorescently labeled. We found that β-actin mRNA in primary fibroblasts localizes predominantly by diffusion and trapping as single mRNAs. In cultured neurons and acute brain slices, wefound that multiple -βactin mRNAs can assemble together, travel by active transport, anddisassemble upon depolarization by potassium chloride. Imaging of brain slices revealed immediate early induction of -βactin transcription after depolarization. Studying endogenous mRNA in live mouse tissues provides insight into its dynamic regulation within the context of the cellular and tissue microenvironment.

UR - http://www.scopus.com/inward/record.url?scp=84892924451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892924451&partnerID=8YFLogxK

U2 - 10.1126/science.1239200

DO - 10.1126/science.1239200

M3 - Article

AN - SCOPUS:84892924451

SN - 0036-8075

VL - 343

SP - 422

EP - 424

JO - Science

JF - Science

IS - 6169

ER -

Visualization of dynamics of single endogenous mRNA labeled in live mouse

Abstract

Publisher link

Find It

Other files and links

Fingerprint

Cite this