Visna DNA synthesis and the tempo of infection in vitro

A. T. Haase, L. Stowring, J. D. Harris, B. Traynor, P. Ventura, R. Peluso, M. Brahic

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Visna virus is the prototype of the subfamily of nontransforming retroviruses that cause slow infections in vivo, and lytic infections in vitro. In this paper we present the first quantitative single cell analysis of the synthesis of viral nucleic acids, using improved methods of in situ hybridization; we identify early visna virus DNA synthesis as the rate-limiting step in transcription, virus production, and cell death in vitro; and we show that by manipulating the extent of early DNA synthesis we can slow the tempo of infection in vitro from 3 days to 3 weeks.

Original languageEnglish (US)
Pages (from-to)399-410
Number of pages12
JournalVirology
Volume119
Issue number2
DOIs
StatePublished - Jun 1982

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