Virus production in microsphere-induced aggregate culture of animal cells

Cheryl M. Perusich; Stephane Goetghebeur; Wei-Shou Hu

doi:10.1007/BF00159987

Virus production in microsphere-induced aggregate culture of animal cells

Cheryl M. Perusich, Stephane Goetghebeur, Wei-Shou Hu

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

Original language	English (US)
Pages (from-to)	145-148
Number of pages	4
Journal	Biotechnology Techniques
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/BF00159987
State	Published - Mar 1991

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title = "Virus production in microsphere-induced aggregate culture of animal cells",

abstract = "Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).",

author = "Perusich, {Cheryl M.} and Stephane Goetghebeur and Wei-Shou Hu",

year = "1991",

month = mar,

doi = "10.1007/BF00159987",

language = "English (US)",

volume = "5",

pages = "145--148",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Springer Netherlands",

number = "2",

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T1 - Virus production in microsphere-induced aggregate culture of animal cells

AU - Perusich, Cheryl M.

AU - Goetghebeur, Stephane

AU - Hu, Wei-Shou

PY - 1991/3

Y1 - 1991/3

N2 - Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

AB - Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

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U2 - 10.1007/BF00159987

DO - 10.1007/BF00159987

M3 - Article

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SP - 145

EP - 148

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 2

ER -

Virus production in microsphere-induced aggregate culture of animal cells

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