Virus production in microsphere-induced aggregate culture of animal cells

Cheryl M. Perusich, Stephane Goetghebeur, Wei-Shou Hu

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalBiotechnology Techniques
Volume5
Issue number2
DOIs
StatePublished - Mar 1 1991

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