Betasatellites are geminivirus-associated single-stranded DNA molecules that play an important role in symptom modulation. A VIGS vector was developed by modifying cotton leaf curl Multan betasatellite (CLCuMB). CLCuMB DNA was modified by replacing the βC1 gene with a multiple cloning site. The silencing ability of the modified CLCuMB was investigated by cloning a fragment of a host gene (Su) or a reporter transgene (uidA) into the modified CLCuMB and co-agroinoculation with cotton leaf curl Multan virus, cotton leaf curl Kokhran virus, and ageratum enation virus, separately. The inoculated Nicotiana tabacum, N. benthamiana, Solanum lycopersicum, Arabidopsis thaliana and Gossypium hirsutum plants showed efficient silencing of the cognate genes.
Bibliographical noteFunding Information:
This work was supported by Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India. SPS gratefully acknowledges the SERB grant (SR/FT/LS-88/2011). JK, SG and JK are thankful to CSIR, and AK to UGC (SAP), for providing fellowships.