Viral RNA is required for the association of APOBEC3G with human immunodeficiency virus type 1 nucleoprotein complexes

Mohammad A. Khan, Sandra Kao, Eri Miyagi, Hiroaki Takeuchi, Ritu Goila-Gaur, Sandrine Opi, Clay L. Gipson, Tristram G. Parslow, Hinh Ly, Klaus Strebel

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144 Scopus citations

Abstract

APOBEC3G (APO3G) is a host cytidine deaminase that is incorporated into human immunodeficiency virus type 1 (HIV-1) particles. We report here that viral RNA promotes stable association of APO3G with HIV-1 nucleoprotein complexes (NPC). A target sequence located within the 5′-untranslated region of the HIV-1 RNA was identified to be necessary and sufficient for efficient APO3G packaging. Fine mapping revealed a sequence normally involved in viral genomic RNA dimerization and Gag binding to be important for APO3G packaging and association with viral NPC. Our data suggest that packaging of APO3G into HIV-1 NPC is enhanced by viral RNA.

Original languageEnglish (US)
Pages (from-to)5870-5874
Number of pages5
JournalJournal of virology
Volume79
Issue number9
DOIs
StatePublished - May 2005

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    Khan, M. A., Kao, S., Miyagi, E., Takeuchi, H., Goila-Gaur, R., Opi, S., Gipson, C. L., Parslow, T. G., Ly, H., & Strebel, K. (2005). Viral RNA is required for the association of APOBEC3G with human immunodeficiency virus type 1 nucleoprotein complexes. Journal of virology, 79(9), 5870-5874. https://doi.org/10.1128/JVI.79.9.5870-5874.2005