Viral IL-10 gene transfer inhibits DTH responses to soluble antigens

Evidence for involvement of genetically modified dendritic cells and macrophages

Janey D. Whalen, Angus W. Thomson, Lina Lu, Paul D Robbins, Christopher H. Evans

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Expression of the viral interleukin-10 (vIL-10) gene within one joint of an animal with polyarticular, inflammatory arthritis suppresses disease in both treated and untreated joints (the "contralateral effect"). We used a mouse delayed-type hypersensitivity (DTH) model to investigate this phenomenon. Adenoviral delivery of the vIL-10 gene suppressed DTH reactions in injected and contralateral paws. T lymphocytes recovered from immunized mice injected with the adenoviral vector (ad-vIL-10) were unable to transfer the DTH response, but were not inhibitory. Peritoneal exudate cells recovered from mice injected intraperitoneally with ad-vIL-10 inhibited DTH reactions in recipient mice, but only when the donor mice had been sensitized to the antigen used to incite the DTH response. Dendritic cells (DCs) recovered from the draining lymph nodes of mice injected with ad-vIL-10 behaved similarly. Bone-marrow-derived DCs cultured ex vivo with ad-vIL-10 or recombinant mouse IL-10 also suppressed DTH reactions by adoptive transfer when pulsed with the inciting antigen. Collectively, these data suggest a mechanism for the contralateral effect in which genetically modified macrophages and DCs present antigen in the context of high, local concentrations of vIL-10, thereby generating unresponsive T lymphocytes. These findings suggest new ways in which to treat immune-driven diseases by gene and cell therapy.

Original languageEnglish (US)
Pages (from-to)543-550
Number of pages8
JournalMolecular Therapy
Volume4
Issue number6
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Fingerprint

Delayed Hypersensitivity
Interleukin-10
Dendritic Cells
Macrophages
Antigens
Genes
Joints
T-Lymphocytes
Adoptive Transfer
Immune System Diseases
Exudates and Transudates
Cell- and Tissue-Based Therapy
Genetic Therapy
Arthritis
Lymph Nodes
Bone Marrow

Keywords

  • Antigen presentation
  • Arthritis
  • Autoimmunity
  • Contralateral effect
  • Dendritic cell
  • Immunomodulation
  • Lymphocyte
  • Macrophage
  • ] Adoptive transfer

Cite this

Viral IL-10 gene transfer inhibits DTH responses to soluble antigens : Evidence for involvement of genetically modified dendritic cells and macrophages. / Whalen, Janey D.; Thomson, Angus W.; Lu, Lina; Robbins, Paul D; Evans, Christopher H.

In: Molecular Therapy, Vol. 4, No. 6, 01.01.2001, p. 543-550.

Research output: Contribution to journalArticle

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