This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.
Bibliographical noteFunding Information:
This study was supported in part by a grant (NASOAA-D00049) from the Office of Marine Pollution Assessment, NOAA, U.S. Department of Commerce, Rockville, MD. Secretarial assistance of Joan Gray is gratefully acknowledged.
- rotavirus marine pollution concentration method human enteric viruses