VIPERdb2: An enhanced and web API enabled relational database for structural virology

Mauricio Carrillo-Tripp, Craig M. Shepherd, Ian A. Borelli, Sangita Venkataraman, Gabriel Lander, Padmaja Natarajan, John E. Johnson, Charles I. Brooks, Vijay S. Reddy

Research output: Contribution to journalArticlepeer-review

309 Scopus citations

Abstract

VIPERdb (http://viperdb.scripps.edu) is a relational database and a web portal for icosahedral virus capsid structures. Our aim is to provide a comprehensive resource specific to the needs of the virology community, with an emphasis on the description and comparison of derived data from structural and computational analyses of the virus capsids. In the current release, VIPERdb2, we implemented a useful and novel method to represent capsid protein residues in the icosahedral asymmetric unit (IAU) using azimuthal polar orthographic projections, otherwise known as Φ-Ψ (Phi-Psi) diagrams. In conjunction with a new Application Programming Interface (API), these diagrams can be used as a dynamic interface to the database to map residues (categorized as surface, interface and core residues) and identify family wide conserved residues including hotspots at the interfaces. Additionally, we enhanced the interactivity with the database by interfacing with web-based tools. In particular, the applications Jmol and STRAP were implemented to visualize and interact with the virus molecular structures and provide sequence-structure alignment capabilities. Together with extended curation practices that maintain data uniformity, a relational database implementation based on a schema for macromolecular structures and the APIs provided will greatly enhance the ability to do structural bioinformatics analysis of virus capsids.

Original languageEnglish (US)
Pages (from-to)D436-D442
JournalNucleic acids research
Volume37
Issue numberSUPPL. 1
DOIs
StatePublished - 2009
Externally publishedYes

Bibliographical note

Funding Information:
VIPERdb is being developed as a training/service and dissemination component of the National Institutes of Health Research Resource: Multiscale Modeling Tools for Structural Biology (MMTSB), which is fully funded by the National Center for Research Resources of the National Institutes of Health (RR12255). Funding for open access charge: NIH, NCRR, RR12255.

Fingerprint

Dive into the research topics of 'VIPERdb2: An enhanced and web API enabled relational database for structural virology'. Together they form a unique fingerprint.

Cite this