TY - JOUR
T1 - Versatile insertion plasmids for targeted genome manipulations in bacteria
T2 - Isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome
AU - Pósfai, György
AU - Koob, Michael D.
AU - Kirkpatrick, Heather A.
AU - Blattner, Frederick R.
PY - 1997/7
Y1 - 1997/7
N2 - A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.
AB - A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.
UR - http://www.scopus.com/inward/record.url?scp=0030860064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030860064&partnerID=8YFLogxK
U2 - 10.1128/jb.179.13.4426-4428.1997
DO - 10.1128/jb.179.13.4426-4428.1997
M3 - Article
C2 - 9209066
AN - SCOPUS:0030860064
SN - 0021-9193
VL - 179
SP - 4426
EP - 4428
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 13
ER -