Vascular biomechanical properties in mice with smooth muscle specific deletion of Ndst1

Neeta Adhikari, Marie Billaud, Marjorie Carlson, Spencer P. Lake, Kim Ramil C. Montaniel, Rod Staggs, Weihua Guan, Dinesha Walek, Snider Desir, Brant E. Isakson, Victor H. Barocas, Jennifer L. Hall

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Heparan sulfate proteoglycans act as co-receptors for many chemokines and growth factors. The sulfation pattern of the heparan sulfate chains is a critical regulatory step affecting the binding of chemokines and growth factors. N-deacetylase-N-sulfotransferase1 (Ndst1) is one of the first enzymes to catalyze sulfation. Previously published work has shown that HSPGs alter tangent moduli and stiffness of tissues and cells. We hypothesized that loss of Ndst1 in smooth muscle would lead to significant changes in heparan sulfate modification and the elastic properties of arteries. In line with this hypothesis, the axial tangent modulus was significantly decreased in aorta from mice lacking Ndst1 in smooth muscle (SM22αcre+Ndst1 -/-, p < 0.05, n = 5). The decrease in axial tangent modulus was associated with a significant switch in myosin and actin types and isoforms expressed in aorta and isolated aortic vascular smooth muscle cells. In contrast, no changes were found in the compliance of smaller thoracodorsal arteries of SM22αcre+Ndst1-/- mice. In summary, the major findings of this study were that targeted ablation of Ndst1 in smooth muscle cells results in altered biomechanical properties of aorta and differential expression of myosin and actin types and isoforms.

Original languageEnglish (US)
Pages (from-to)225-238
Number of pages14
JournalMolecular and cellular biochemistry
Volume385
Issue number1-2
DOIs
StatePublished - Jan 1 2014

Keywords

  • Arterial compliance
  • Biomechanics
  • Heparan sulfate proteoglycan
  • Ndst1
  • Tangent moduli

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