TY - JOUR
T1 - Variations in native protein glycation and plasma antioxidants in several birds of prey
AU - Ingram, Tana
AU - Zuck, Jessica
AU - Borges, Chad R.
AU - Redig, Patrick
AU - Sweazea, Karen L.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/8
Y1 - 2017/8
N2 - Birds are an anomaly among vertebrates as they are remarkably long-lived despite having naturally high blood glucose and metabolic rates. For mammals, hyperglycemia leads to oxidative stress and protein glycation. In contrast, many studies have shown that domestic and wild birds are relatively resistant to these glucose-mediated pathologies. Surprisingly very little research has examined protein glycation in birds of prey, which by nature consume a diet high in protein and fat that promotes gluconeogenesis. The purpose of this study was to evaluate protein glycation and antioxidant concentrations in serum samples from several birds of prey (bald eagle (BAEA), red-tailed hawk (RTHA), barred owl (BAOW), great horned owl (GHOW)) as protein glycation can accelerate oxidative stress and vice versa. Serum glucose was measured using a commercially available assay, native albumin glycation was measured by mass spectrometry and various antioxidants (uric acid, vitamin E, retinol and several carotenoids) were measured by high performance liquid chromatography. Although glucose concentrations were not significantly different between species (p = 0.340), albumin glycation was significantly higher (p = 0.004) in BAEA (23.67 ± 1.90%) and BAOW (24.28 ± 1.43%) compared to RTHA (14.31 ± 0.63%). Of the antioxidants examined, lutein was significantly higher in BAOW (p = 0.008). BAEA had the highest beta-cryptoxanthin and beta-carotene concentrations (p < 0.005). The high concentrations of antioxidants in these birds of prey relative to other birds likely helps protect from complications that may otherwise arise from having high glucose and protein glycation.
AB - Birds are an anomaly among vertebrates as they are remarkably long-lived despite having naturally high blood glucose and metabolic rates. For mammals, hyperglycemia leads to oxidative stress and protein glycation. In contrast, many studies have shown that domestic and wild birds are relatively resistant to these glucose-mediated pathologies. Surprisingly very little research has examined protein glycation in birds of prey, which by nature consume a diet high in protein and fat that promotes gluconeogenesis. The purpose of this study was to evaluate protein glycation and antioxidant concentrations in serum samples from several birds of prey (bald eagle (BAEA), red-tailed hawk (RTHA), barred owl (BAOW), great horned owl (GHOW)) as protein glycation can accelerate oxidative stress and vice versa. Serum glucose was measured using a commercially available assay, native albumin glycation was measured by mass spectrometry and various antioxidants (uric acid, vitamin E, retinol and several carotenoids) were measured by high performance liquid chromatography. Although glucose concentrations were not significantly different between species (p = 0.340), albumin glycation was significantly higher (p = 0.004) in BAEA (23.67 ± 1.90%) and BAOW (24.28 ± 1.43%) compared to RTHA (14.31 ± 0.63%). Of the antioxidants examined, lutein was significantly higher in BAOW (p = 0.008). BAEA had the highest beta-cryptoxanthin and beta-carotene concentrations (p < 0.005). The high concentrations of antioxidants in these birds of prey relative to other birds likely helps protect from complications that may otherwise arise from having high glucose and protein glycation.
KW - Albumin
KW - Antioxidant
KW - Bird of prey
KW - Glycation
KW - Raptor
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U2 - 10.1016/j.cbpb.2017.05.004
DO - 10.1016/j.cbpb.2017.05.004
M3 - Article
C2 - 28529085
AN - SCOPUS:85019562038
SN - 0305-0491
VL - 210
SP - 18
EP - 28
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ER -