Variations in ADP-ribosylation of nuclear scaffold proteins during the Hela cell cycle

Kenneth W. Adolph, Min Kyung H Song

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Cell cycle variations in ADP-ribosylation of nuclear scaffold proteins were determined. Nuclei of synchronized cells were isolated and labeled with [32P]NAD before nuclear scaffolds were obtained by digestion of DNA with DNase I and extraction of proteins with 2M NaCl. Autoradiograms revealed the three groups of "lamins" and a species identified as poly(ADP-ribose) polymerase to be the primary ADP-ribosylated proteins. The patterns of modification of nuclear scaffold proteins displayed similar features through the cell cycle. Radioactivity in the lamins increased from 20% in early-S phase to 40% in G1 phase of the next cell cycle.

Original languageEnglish (US)
Pages (from-to)840-847
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume126
Issue number2
DOIs
StatePublished - Jan 31 1985

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