TY - JOUR
T1 - Variation of the meiotic recombination landscape and properties over a broad evolutionary distance in yeasts
AU - Brion, Christian
AU - Legrand, Sylvain
AU - Peter, Jackson
AU - Caradec, Claudia
AU - Pflieger, David
AU - Hou, Jing
AU - Friedrich, Anne
AU - Llorente, Bertrand
AU - Schacherer, Joseph
N1 - Publisher Copyright:
© 2017 Brion et al.
PY - 2017/8
Y1 - 2017/8
N2 - Meiotic recombination is a major factor of genome evolution, deeply characterized in only a few model species, notably the yeast Saccharomyces cerevisiae. Consequently, little is known about variations of its properties across species. In this respect, we explored the recombination landscape of Lachancea kluyveri, a protoploid yeast species that diverged from the Saccharomyces genus more than 100 million years ago and we found striking differences with S. cerevisiae. These variations include a lower recombination rate, a higher frequency of chromosomes segregating without any crossover and the absence of recombination on the chromosome arm containing the sex locus. In addition, although well conserved within the Saccharomyces clade, the S. cerevisiae recombination hotspots are not conserved over a broader evolutionary distance. Finally and strikingly, we found evidence of frequent reversal of commitment to meiosis, resulting in return to mitotic growth after allele shuffling. Identification of this major but underestimated evolutionary phenomenon illustrates the relevance of exploring non-model species.
AB - Meiotic recombination is a major factor of genome evolution, deeply characterized in only a few model species, notably the yeast Saccharomyces cerevisiae. Consequently, little is known about variations of its properties across species. In this respect, we explored the recombination landscape of Lachancea kluyveri, a protoploid yeast species that diverged from the Saccharomyces genus more than 100 million years ago and we found striking differences with S. cerevisiae. These variations include a lower recombination rate, a higher frequency of chromosomes segregating without any crossover and the absence of recombination on the chromosome arm containing the sex locus. In addition, although well conserved within the Saccharomyces clade, the S. cerevisiae recombination hotspots are not conserved over a broader evolutionary distance. Finally and strikingly, we found evidence of frequent reversal of commitment to meiosis, resulting in return to mitotic growth after allele shuffling. Identification of this major but underestimated evolutionary phenomenon illustrates the relevance of exploring non-model species.
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U2 - 10.1371/journal.pgen.1006917
DO - 10.1371/journal.pgen.1006917
M3 - Article
C2 - 28763437
AN - SCOPUS:85028804401
SN - 1553-7390
VL - 13
JO - PLoS genetics
JF - PLoS genetics
IS - 8
M1 - e1006917
ER -