Variation at the b mating type locus of Ustilago maydis

Paul Zambino, James V. Groth, Lewis Lukens, James R. Garton, Georgiana May

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Abstract

Population level diversity at the Ustilago maydis b mating type locus was determined in samples from four Minnesota locations using a combination of plate mating techniques and a polymerase chain reaction (PCR)-based assay. The PCR method allows rapid identification of b types from samples of natural populations and utilizes the hypervariable regions of the b locus that determine mating type specificity. Results demonstrated high levels of b diversity within populations, with one population yielding 17 of the total 18 b types found in the study. Pairwise G(ST) values were in the range of 0.02 to 0.05, and common b mating types were found across broad geographic distances. These data demonstrated that very low levels of differentiation among U. maydis populations occur with respect to b locus variation. Consistent with frequency-dependent selection models, b types were represented at approximately equal frequencies within the entire Minnesota population. However, neutral evolutionary models for patterns of geographic distribution and variation at b cannot be entirely excluded. The importance to agricultural practices of understanding population genetic processes is discussed.

Original languageEnglish (US)
Pages (from-to)1233-1239
Number of pages7
JournalPhytopathology
Volume87
Issue number12
DOIs
StatePublished - Dec 1997

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Keywords

  • Barcode
  • Basidiomycete
  • Corn smut
  • DNA fingerprint
  • Mating system

Cite this

Zambino, P., Groth, J. V., Lukens, L., Garton, J. R., & May, G. (1997). Variation at the b mating type locus of Ustilago maydis. Phytopathology, 87(12), 1233-1239. https://doi.org/10.1094/PHYTO.1997.87.12.1233