Variability of morphology, parasitism, and nucleotide sequences among isolates and species of nematophagous Hirsutella

Mei Chun Xiang; Xiao Hu Yang; Zhe Xiao Wang; Xing Zhong Liu; Sen Yu Chen; Qi Ming Xiao

doi:10.1016/j.biocontrol.2006.12.016

Variability of morphology, parasitism, and nucleotide sequences among isolates and species of nematophagous Hirsutella

Mei Chun Xiang, Xiao Hu Yang, Zhe Xiao Wang, Xing Zhong Liu, Sen Yu Chen, Qi Ming Xiao

Southern Research and Outreach Center

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Three nematode-endoparasitic, Hirsutella rhossiliensis (18 isolates), Hirsutella minnesotensis (8 isolates) and Hirsutella vermicola (3 isolates) were studied for their variability in morphology, nematode parasitism, and DNA sequences of internal transcribed spacer (ITS) of ribosomal DNA (rDNA) and mitogen-activated protein kinase (MAPK) gene fragments. There were differences in length and width of conidiogenous cells and conidia among the three Hirsutella species and among isolates within species of H. rhossiliensis and H. minnesotensis. Most isolates of H. rhossiliensis and H. minnesotensis parasitized higher percentages of the cyst nematodes, Heterodera glycines and Heterodera avenae, than the four non-cyst nematodes, Meloidogyne hapla, Bursaphelenchus xylophilus, Heterorhabditis bacteriophora, and Steinernema carpocapsae. H. vermicola had no or weak parasitism on the six assayed nematode species. The phylogenetic trees of ITS region, MAPK gene fragments and their combination revealed that there was genetic variability among species and isolates, but there was no apparent relationship between the genetic variability and their host or geographic origin.

Original language	English (US)
Pages (from-to)	110-119
Number of pages	10
Journal	Biological Control
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.biocontrol.2006.12.016
State	Published - Apr 2007

Bibliographical note

Funding Information:
We thank R.A. Humber for providing the isolates which from the USDA-ARS Collection of Entomopathogenic Fungal Cultures; D.L. Peng, B.J. Yang, and H. Jian for providing H. avenae, B. xylophilus, and entomopathogenic nematodes, respectively; and R. Ma for her critical review of the manuscript. This research was jointly supported by National Natural Scientific Foundation of China (No. 30370953 and 30625001) and Beijing Municipal Science & Technology Commission (D0706005040331) and D0705002040191.

Keywords

Biological control
Hirsutella minnesotensis
Hirsutella rhossiliensis
Hirsutella vermicola
Nematode
Parasitism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biocontrol.2006.12.016

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title = "Variability of morphology, parasitism, and nucleotide sequences among isolates and species of nematophagous Hirsutella",

abstract = "Three nematode-endoparasitic, Hirsutella rhossiliensis (18 isolates), Hirsutella minnesotensis (8 isolates) and Hirsutella vermicola (3 isolates) were studied for their variability in morphology, nematode parasitism, and DNA sequences of internal transcribed spacer (ITS) of ribosomal DNA (rDNA) and mitogen-activated protein kinase (MAPK) gene fragments. There were differences in length and width of conidiogenous cells and conidia among the three Hirsutella species and among isolates within species of H. rhossiliensis and H. minnesotensis. Most isolates of H. rhossiliensis and H. minnesotensis parasitized higher percentages of the cyst nematodes, Heterodera glycines and Heterodera avenae, than the four non-cyst nematodes, Meloidogyne hapla, Bursaphelenchus xylophilus, Heterorhabditis bacteriophora, and Steinernema carpocapsae. H. vermicola had no or weak parasitism on the six assayed nematode species. The phylogenetic trees of ITS region, MAPK gene fragments and their combination revealed that there was genetic variability among species and isolates, but there was no apparent relationship between the genetic variability and their host or geographic origin.",

keywords = "Biological control, Hirsutella minnesotensis, Hirsutella rhossiliensis, Hirsutella vermicola, Nematode, Parasitism",

author = "Xiang, {Mei Chun} and Yang, {Xiao Hu} and Wang, {Zhe Xiao} and Liu, {Xing Zhong} and Chen, {Sen Yu} and Xiao, {Qi Ming}",

note = "Funding Information: We thank R.A. Humber for providing the isolates which from the USDA-ARS Collection of Entomopathogenic Fungal Cultures; D.L. Peng, B.J. Yang, and H. Jian for providing H. avenae, B. xylophilus, and entomopathogenic nematodes, respectively; and R. Ma for her critical review of the manuscript. This research was jointly supported by National Natural Scientific Foundation of China (No. 30370953 and 30625001) and Beijing Municipal Science & Technology Commission (D0706005040331) and D0705002040191.",

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AU - Chen, Sen Yu

AU - Xiao, Qi Ming

N1 - Funding Information: We thank R.A. Humber for providing the isolates which from the USDA-ARS Collection of Entomopathogenic Fungal Cultures; D.L. Peng, B.J. Yang, and H. Jian for providing H. avenae, B. xylophilus, and entomopathogenic nematodes, respectively; and R. Ma for her critical review of the manuscript. This research was jointly supported by National Natural Scientific Foundation of China (No. 30370953 and 30625001) and Beijing Municipal Science & Technology Commission (D0706005040331) and D0705002040191.

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N2 - Three nematode-endoparasitic, Hirsutella rhossiliensis (18 isolates), Hirsutella minnesotensis (8 isolates) and Hirsutella vermicola (3 isolates) were studied for their variability in morphology, nematode parasitism, and DNA sequences of internal transcribed spacer (ITS) of ribosomal DNA (rDNA) and mitogen-activated protein kinase (MAPK) gene fragments. There were differences in length and width of conidiogenous cells and conidia among the three Hirsutella species and among isolates within species of H. rhossiliensis and H. minnesotensis. Most isolates of H. rhossiliensis and H. minnesotensis parasitized higher percentages of the cyst nematodes, Heterodera glycines and Heterodera avenae, than the four non-cyst nematodes, Meloidogyne hapla, Bursaphelenchus xylophilus, Heterorhabditis bacteriophora, and Steinernema carpocapsae. H. vermicola had no or weak parasitism on the six assayed nematode species. The phylogenetic trees of ITS region, MAPK gene fragments and their combination revealed that there was genetic variability among species and isolates, but there was no apparent relationship between the genetic variability and their host or geographic origin.

AB - Three nematode-endoparasitic, Hirsutella rhossiliensis (18 isolates), Hirsutella minnesotensis (8 isolates) and Hirsutella vermicola (3 isolates) were studied for their variability in morphology, nematode parasitism, and DNA sequences of internal transcribed spacer (ITS) of ribosomal DNA (rDNA) and mitogen-activated protein kinase (MAPK) gene fragments. There were differences in length and width of conidiogenous cells and conidia among the three Hirsutella species and among isolates within species of H. rhossiliensis and H. minnesotensis. Most isolates of H. rhossiliensis and H. minnesotensis parasitized higher percentages of the cyst nematodes, Heterodera glycines and Heterodera avenae, than the four non-cyst nematodes, Meloidogyne hapla, Bursaphelenchus xylophilus, Heterorhabditis bacteriophora, and Steinernema carpocapsae. H. vermicola had no or weak parasitism on the six assayed nematode species. The phylogenetic trees of ITS region, MAPK gene fragments and their combination revealed that there was genetic variability among species and isolates, but there was no apparent relationship between the genetic variability and their host or geographic origin.

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VL - 41

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JO - Biological Control

JF - Biological Control

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ER -

Variability of morphology, parasitism, and nucleotide sequences among isolates and species of nematophagous Hirsutella

Abstract

Bibliographical note

Keywords

UN SDGs

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