CONTEXT.—: The measurement of cytokines in clinical laboratories is becoming an increasingly routine part of immune monitoring when administering biologic and cell-based immunotherapies and also for clinical assessment of inflammatory conditions. While a number of commercial assays and platforms are available for cytokine measurement, there is currently little standardization among these analytical methods.
OBJECTIVE.—: To characterize the variability and comparability among cytokine testing platforms that are commonly used in clinical laboratories.
DESIGN.—: We analyzed data for 4 cytokines (interleukin [IL]-1, IL-6, IL-8, and tumor necrosis factor-alpha [TNF-α]) from 6 College of American Pathologists cytokine surveys administered from 2015 to 2018. Analyses interrogated variability between testing methods and variability within each laboratory across the mailings.
RESULTS.—: Significant variability was noted across methods with analysis of IL-1 showing the least variability and IL-6, IL-8, and TNF-α varying between methods to a greater extent. Intralab variability was also significant with TNF-α measurements again showing the greatest variability.
CONCLUSIONS.—: This retrospective analysis of College of American Pathologists proficiency testing data for cytokine measurement is the largest method comparison to date, and this study provides a description of the variation of cytokine measurement across methods, across laboratories, and within laboratories. Serial monitoring of cytokines should preferentially be performed by the same method within the same laboratory.
|Original language||English (US)|
|Number of pages||4|
|Journal||Archives of Pathology and Laboratory Medicine|
|State||Published - Oct 2020|
Bibliographical notePublisher Copyright:
© 2020 College of American Pathologists. All rights reserved.
- Laboratory Proficiency Testing/standards
- Pathology, Clinical/standards
- Quality Improvement
- Retrospective Studies
PubMed: MeSH publication types
- Journal Article