TY - JOUR
T1 - Vacuolar glyphosate-sequestration correlates with glyphosate resistance in ryegrass (Lolium spp.) from Australia, South America, and Europe
T2 - A 31P NMR investigation
AU - Ge, Xia
AU - D'Avignon, D. André
AU - Ackerman, Joseph J.H.
AU - Collavo, Alberto
AU - Sattin, Maurizio
AU - Ostrander, Elizabeth L.
AU - Hall, Erin L.
AU - Sammons, R. Douglas
AU - Preston, Christopher
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/2/8
Y1 - 2012/2/8
N2 - Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD 50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC 50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD 50 and IC 50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by 31P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous 31P NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.
AB - Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD 50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC 50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD 50 and IC 50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by 31P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous 31P NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.
KW - Lolium spp.
KW - glyphosate
KW - glyphosate resistance
KW - glyphosate tonoplast transporter
KW - in vivo 31P NMR
UR - http://www.scopus.com/inward/record.url?scp=84856979150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856979150&partnerID=8YFLogxK
U2 - 10.1021/jf203472s
DO - 10.1021/jf203472s
M3 - Article
C2 - 22224711
AN - SCOPUS:84856979150
SN - 0021-8561
VL - 60
SP - 1243
EP - 1250
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 5
ER -
