Vaccinia virus transfection of hippocampal slice neurons

D. L. Pettit, Thillai Koothan, Dezhi Liao, R. Malinow

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.

Original languageEnglish (US)
Pages (from-to)685-688
Number of pages4
JournalNeuron
Volume14
Issue number4
DOIs
StatePublished - Apr 1995

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