Vaccinia virus transfection of hippocampal slice neurons

D. L. Pettit; Thillai Koothan; Dezhi Liao; R. Malinow

doi:10.1016/0896-6273(95)90212-0

Vaccinia virus transfection of hippocampal slice neurons

D. L. Pettit, Thillai Koothan, Dezhi Liao, R. Malinow

Neuroscience

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.

Original language	English (US)
Pages (from-to)	685-688
Number of pages	4
Journal	Neuron
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/0896-6273(95)90212-0
State	Published - Apr 1995

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title = "Vaccinia virus transfection of hippocampal slice neurons",

abstract = "Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.",

author = "Pettit, {D. L.} and Thillai Koothan and Dezhi Liao and R. Malinow",

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AU - Pettit, D. L.

AU - Koothan, Thillai

AU - Liao, Dezhi

AU - Malinow, R.

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AB - Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.

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Vaccinia virus transfection of hippocampal slice neurons

Abstract

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