Using NextRAD sequencing to infer movement of herbivores among host plants

Zhen Fu, Brendan Epstein, Joanna L. Kelley, Qi Zheng, Alan O. Bergland, Carmen I. Castillo Carrillo, Andrew S. Jensen, Jennifer Dahan, Alexander V. Karasev, William E. Snyder

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Herbivores often move among spatially interspersed host plants, tracking high-quality resources through space and time. This dispersal is of particular interest for vectors of plant pathogens. Existing molecular tools to track such movement have yielded important insights, but often provide insufficient genetic resolution to infer spread at finer spatiotemporal scales. Here, we explore the use of Nextera-tagmented reductively-amplified DNA (NextRAD) sequencing to infer movement of a highly-mobile winged insect, the potato psyllid (Bactericera cockerelli), among host plants. The psyllid vectors the pathogen that causes zebra chip disease in potato (Solanum tuberosum), but understanding and managing the spread of this pathogen is limited by uncertainty about the insect's host plant(s) outside of the growing season. We identified 1,978 polymorphic loci among psyllids separated spatiotemporally on potato or in patches of bittersweet nightshade (S. dulcumara), a weedy plant proposed to be the source of potato-colonizing psyllids. A subset of the psyllids on potato exhibited genetic similarity to insects on nightshade, consistent with regular movement between these two host plants. However, a second subset of potato-collected psyllids was genetically distinct from those collected on bittersweet nightshade; this suggests that a currently unrecognized source, i.e., other nightshade patches or a third host-plant species, could be contributing to psyllid populations in potato. Oftentimes, dispersal of vectors of pathogens must be tracked at a fine scale in order to understand, predict, and manage disease spread. We demonstrate that emerging sequencing technologies that detect genomewide SNPs of a vector can be used to infer such localized movement.

Original languageEnglish (US)
Article number0177742
JournalPloS one
Volume12
Issue number5
DOIs
StatePublished - May 2017

Bibliographical note

Funding Information:
This research was funded by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Specialty Crop Research Initiative (https://nifa.usda.gov/funding-opportunity/ specialty-crop-research-initiative-scri) award number 2015-09273, along with grants from the Northwest Potato Research Consortium (http:// www.nwpotatoresearch.com/) and the Washington State Commission on Pesticide Registration (http:// wscpr.org/), all to WES. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Fingerprint Dive into the research topics of 'Using NextRAD sequencing to infer movement of herbivores among host plants'. Together they form a unique fingerprint.

Cite this