Using network analysis to explore co-occurrence patterns in soil microbial communities

Albert Barberán, Scott T. Bates, Emilio O. Casamayor, Noah Fierer

Research output: Contribution to journalArticlepeer-review

804 Scopus citations

Abstract

Exploring large environmental datasets generated by high-throughput DNA sequencing technologies requires new analytical approaches to move beyond the basic inventory descriptions of the composition and diversity of natural microbial communities. In order to investigate potential interactions between microbial taxa, network analysis of significant taxon co-occurrence patterns may help to decipher the structure of complex microbial communities across spatial or temporal gradients. Here, we calculated associations between microbial taxa and applied network analysis approaches to a 16S rRNA gene barcoded pyrosequencing dataset containing >160 000 bacterial and archaeal sequences from 151 soil samples from a broad range of ecosystem types. We described the topology of the resulting network and defined operational taxonomic unit categories based on abundance and occupancy (that is, habitat generalists and habitat specialists). Co-occurrence patterns were readily revealed, including general non-random association, common life history strategies at broad taxonomic levels and unexpected relationships between community members. Overall, we demonstrated the potential of exploring inter-taxa correlations to gain a more integrated understanding of microbial community structure and the ecological rules guiding community assembly.

Original languageEnglish (US)
Pages (from-to)343-351
Number of pages9
JournalISME Journal
Volume6
Issue number2
DOIs
StatePublished - Feb 2012

Bibliographical note

Funding Information:
and members of the Rob Knight lab in UC, particularly Greg Caporaso for computational support and the development of QIIME. AB is supported by the Spanish FPU predoctoral scholarship program and EOC by grants PIRENA CGL2009-13318 and CONSOLIDER-INGENIO 2010 GRACCIE CSD2007-00067 from the Spanish Ministerio de Ciencia e Innovación (MICINN).

Keywords

  • 16S rRNA gene
  • co-occurrence
  • community ecology
  • network analysis
  • pyrosequencing
  • soil

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