Using microsatellite DNA to determine whether American Hazelnut Clumps are multiclonal

Michael Demchik, Jason Fischbach, Anthony Kern, Keith Turnquist, Isaac Palmer

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


We have been selecting high yielding American hazelnut (Corylus americana) from wild populations. Because hazelnuts tend to have a spreading growth form, it was unclear whether phenotypic variation among the individual stems of a clump was due to multiple genotypes which had grown together. This would significantly complicate selection. In order to determine if these bushes were single genotypes, we collected leaf samples from the four cardinal directions from each of 23 high producing stems. These were analyzed for 4–6 microsatellite loci. We determined that of the 23 selections, 5 were composed of more than one genotype. Additionally, one clump had one allele on one direction that was different (likely allele dropout from a heterozygote where one allele did not amplify correctly). Overall, the majority of clumps (78 %) were composed of a single genotype. This suggests that selecting at the level of “clump” is viable. Additionally, we noticed that within our selection sites, certain plants seemed to function only as males (produced male catkins but seldom produced female flowers) while others produced copious female flowers and subsequently nuts. Within one site, we selected 20 hazelnuts with both male and female flowers and 19 with only male flowers. Using the same microsatellite techniques for 10 loci, we assessed whether there were obvious genetic differences between these types of plants. Overall, there was no difference between plants that bore female flowers and those that did not. Microsatellite DNA techniques are becoming a more useful tool for selection of potential agroforestry crops from wild populations.

Original languageEnglish (US)
Pages (from-to)927-931
Number of pages5
JournalAgroforestry Systems
Issue number5
StatePublished - Oct 1 2016
Externally publishedYes

Bibliographical note

Funding Information:
This project was funded in part by the University of Wisconsin Stevens Point University Professional Development Committee and the Wisconsin Institute for Sustainable Technology. Numerous students were involved in the collection and processing of these samples: some include Kevin Schwartz, Helana Nelson, Emily and Benjamin Demchik, and others. Sophie Demchik and two anonymous reviewers provided review of the paper. Various state and federal land managers provided site access.

Publisher Copyright:
© 2016, Springer Science+Business Media Dordrecht.


  • Allele
  • Corylus
  • SSR
  • Selection


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