Using Microinjection of Mammalian Cultured Cells to Study Cell Division

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The introduction of macromolecules directly into individual cells by microinjection is an important technique for manipulating mitotic cells. mRNA, purified proteins, or concentrated antibodies can all be injected directly into a single cell, and their effects monitored by live-cell imaging. The equipment necessary is relatively simple, and the technique can be easily mastered. Here we describe our microinjection setup, how to microinject cultured mammalian cells in mitosis, and how to analyze those cells by same-cell live and fixed imaging.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages105-122
Number of pages18
Volume2415
DOIs
StatePublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Antibody
  • Cell cycle
  • Fluorescence
  • Injector
  • Micromanipulator
  • Microneedle
  • Microscopy
  • Mitosis
  • Phosphorylation

PubMed: MeSH publication types

  • Journal Article

Fingerprint

Dive into the research topics of 'Using Microinjection of Mammalian Cultured Cells to Study Cell Division'. Together they form a unique fingerprint.

Cite this