The introduction of macromolecules directly into individual cells by microinjection is an important technique for manipulating mitotic cells. mRNA, purified proteins, or concentrated antibodies can all be injected directly into a single cell, and their effects monitored by live-cell imaging. The equipment necessary is relatively simple, and the technique can be easily mastered. Here we describe our microinjection setup, how to microinject cultured mammalian cells in mitosis, and how to analyze those cells by same-cell live and fixed imaging.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||18|
|State||Published - 2022|
|Name||Methods in molecular biology (Clifton, N.J.)|
Bibliographical notePublisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
- Cell cycle
PubMed: MeSH publication types
- Journal Article