Using embryonic stem cells as a model of pancreatic development

Research output: ResearchChapter

Abstract

Embryonic stem cells, which can differentiate into insulin-producing cells and express pancreas specific markers during development in vitro, provide a model for studying pancreatic development. Current models of pancreatic development are based on animal models, including zebrafish, chicken and mouse. Genetic manipulations in the mouse have been a major source of information to date.More recently, in vitro differentiation of mouse embryonic stem cells has been used to allow both environmental and genetic manipulation during differentiation.While gene expression during in vitro differentiation appears to reproduce patterns found in vivo, comparison of mouse and human embryonic stem cell differentiation has demonstrated differences between regulation of mouse and human development. In this chapter, we review several studies on ES cell differentiation into insulinproducing cells in vitro and analysis of pancreatic-specific gene expression during differentiation, and discuss the potential of using human ES cells as a model of human pancreatic development.

LanguageEnglish (US)
Title of host publicationStem Cells and Regenerative Medicine
PublisherWorld Scientific Publishing Co.
Pages27-44
Number of pages18
ISBN (Print)9789812775771, 9812775765, 9789812775764
DOIs
StatePublished - Jan 1 2008

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Embryonic Stem Cells
In Vitro Techniques
Stem cells
Human Development
Cell Differentiation
Gene Expression
Mouse Embryonic Stem Cells
Gene expression
Zebrafish
Pancreas
Chickens
Animal Models
Insulin
Human Embryonic Stem Cells
Animals

Cite this

Geng, Z., Chase, L., & Firpo, M. T. (2008). Using embryonic stem cells as a model of pancreatic development. In Stem Cells and Regenerative Medicine (pp. 27-44). World Scientific Publishing Co.. DOI: 10.1142/9789812775771_0002

Using embryonic stem cells as a model of pancreatic development. / Geng, Zhaohui; Chase, Lucas; Firpo, Meri T.

Stem Cells and Regenerative Medicine. World Scientific Publishing Co., 2008. p. 27-44.

Research output: ResearchChapter

Geng, Z, Chase, L & Firpo, MT 2008, Using embryonic stem cells as a model of pancreatic development. in Stem Cells and Regenerative Medicine. World Scientific Publishing Co., pp. 27-44. DOI: 10.1142/9789812775771_0002
Geng Z, Chase L, Firpo MT. Using embryonic stem cells as a model of pancreatic development. In Stem Cells and Regenerative Medicine. World Scientific Publishing Co.2008. p. 27-44. Available from, DOI: 10.1142/9789812775771_0002
Geng, Zhaohui ; Chase, Lucas ; Firpo, Meri T./ Using embryonic stem cells as a model of pancreatic development. Stem Cells and Regenerative Medicine. World Scientific Publishing Co., 2008. pp. 27-44
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