The design of a two-phase-flow (water in oil) microfluidic PCR svstem led us to solve different problems in order to achieve DNA amplification: droplet train instability, cross contamination between droplets, being able to coalesce two droplets without direct contact with them. After a short explanation of the PCR principle, the experimental conditions used to achieve droplets train stability and avoid contamination between droplets will be presented, as well as the amplification results obtained. We will then present a system based on the electrocoalescence principle which allows to coalesce without direct contact two water droplets inside the capillary.
|Translated title of the contribution||Using droplet microfluidics to design a continuous flow PCR system|
|Number of pages||6|
|State||Published - Nov 21 2006|