Using dendritic cell maturation and IL-12 producing capacity as markers of function

A cautionary tale

Anjum S Kaka, Aaron E. Foster, Heidi L. Weiss, Cliona M. Rooney, Ann M. Leen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Effective dendritic cell (DC) function depends on sufficient expression of antigen and costimulatory molecules, and secretion of interleukin (IL)-12. We sought to augment DC stimulatory capacity by optimizing DC phenotype and IL-12 production. DCs, obtained by CD14-selection, were matured using 8 different cytokine cocktails, and expression of costimulatory/major histocompatibility complex molecules and IL-12 production at the end of maturation was assessed. DC stimulatory capacity was determined after pulsing with immunogenic adenoviral CD8 peptide epitopes or after transduction with an Ad5f35-null vector. Resultant T-cell cultures were analyzed using pentamer and interferon-γ enzyme-linked immunosorbent spot assays. On the basis of DC expression of maturation markers and IL-12 production, we defined prototype "minimal" [tumor necrosis factor-α (TNF-α), prostaglandin E2], "standard" (IL-1, IL-6, TNF-α, prostaglandin E2), and "optimal" (IL-1, IL-6, TNF-α, interferon-α, CD40 ligand) DC cocktails. Optimal DCs were functionally superior when pulsed with CD8 peptides, but when transduced with Ad5f35, functioned poorly as antigen-presenting cells. We investigated the mechanisms underlying this discrepancy and suggest that prolonged stimulation with potent cytokines (optimal cocktail) in combination with adenoviral transduction alters the kinetics of DC maturation such that the DCs are functionally exhausted by the traditional 48-hour maturation time point. Shortening the DC maturation period posttransduction restored optimal DC stimulatory capacity. Thus, maturation stimuli and viral transduction affects DC phenotype, IL-12 producing capacity, and kinetics of maturation, and all must be considered before designing protocols to generate the optimal DC for cytotoxic T lymphocyte generation.

Original languageEnglish (US)
Pages (from-to)359-369
Number of pages11
JournalJournal of Immunotherapy
Volume31
Issue number4
DOIs
StatePublished - May 1 2008

Fingerprint

Interleukin-12
Dendritic Cells
Tumor Necrosis Factor-alpha
Interleukin-1
Dinoprostone
Interferons
Interleukin-6
Cytokines
Phenotype
CD40 Ligand
Peptides
Cytotoxic T-Lymphocytes
Antigen-Presenting Cells
Major Histocompatibility Complex
Epitopes
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Antigens

Keywords

  • Adenovirus
  • CTL
  • Dendritic cell
  • IL-12
  • Maturation cocktail

Cite this

Using dendritic cell maturation and IL-12 producing capacity as markers of function : A cautionary tale. / Kaka, Anjum S; Foster, Aaron E.; Weiss, Heidi L.; Rooney, Cliona M.; Leen, Ann M.

In: Journal of Immunotherapy, Vol. 31, No. 4, 01.05.2008, p. 359-369.

Research output: Contribution to journalArticle

Kaka, Anjum S ; Foster, Aaron E. ; Weiss, Heidi L. ; Rooney, Cliona M. ; Leen, Ann M. / Using dendritic cell maturation and IL-12 producing capacity as markers of function : A cautionary tale. In: Journal of Immunotherapy. 2008 ; Vol. 31, No. 4. pp. 359-369.
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