The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
|Original language||English (US)|
|Number of pages||9|
|Journal||Plant Biotechnology Journal|
|State||Published - Jan 2019|
Bibliographical noteFunding Information:
This work was supported by grants from Bill and Melinda Gates Foundation (Grand Challenges Exploration, grant number OPP1060026), Biotechnology and Biological Sciences Research Council (BBSRC) BB/L009293/1), US Israel Binational Agriculture Research and Development Fund to N.O. (grant no. US-4813-15C) and Gatsby Charitable Foundation. We are also grateful to Liz Hughes and Neil Stammars for their technical help.
© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
- gibberellic acid