Abstract
The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 132-140 |
| Number of pages | 9 |
| Journal | Plant Biotechnology Journal |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2019 |
Bibliographical note
Funding Information:This work was supported by grants from Bill and Melinda Gates Foundation (Grand Challenges Exploration, grant number OPP1060026), Biotechnology and Biological Sciences Research Council (BBSRC) BB/L009293/1), US Israel Binational Agriculture Research and Development Fund to N.O. (grant no. US-4813-15C) and Gatsby Charitable Foundation. We are also grateful to Liz Hughes and Neil Stammars for their technical help.
Publisher Copyright:
© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Keywords
- CRISPR/Cas9
- DELLA
- PROCERA
- dwarf
- gibberellic acid
- slender