Using an F2 Screen to Search for Resistance Alleles to Bacillus thuringiensis Toxin in European Corn Borer (Lepidoptera: Crambidae)

D. A. Andow, D. N. Alstad, Y. H. Pang, P. C. Bolin, W. D. Hutchison

Research output: Contribution to journalReview article

81 Scopus citations

Abstract

We present an application of an F2 screening method for recovering and estimating the frequencies of rare alleles that confer insect resistance to a transgenic corn variety producing Bacillus thuringiensis Berliner crystal protein toxin (Bt corn). Based on a sample of 91 female Ostrinia nubilalis (Hübner) we show with 95% confidence that the frequency of B. thuringiensis resistance alleles from a wild Minnesota population is <0.013. This is an upper limit to the estimated allele frequency and does not provide clear evidence that 1 of the assumptions of the refuge plus high-dose strategy will or will not be met. With additional sampling, a more precise estimate of resistance allele frequency could be obtained that would clearly support or refute 1 of the assumptions of the refuge plus high-dose strategy. Variable costs of the screening method were $19.70 per female line, but these could be reduced by improved collecting, rearing, and handling methods.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalJournal of economic entomology
Volume91
Issue number3
DOIs
StatePublished - Jun 1998

Keywords

  • Allele frequency
  • Bacillus thuringiensis
  • European corn borer
  • Monitoring
  • Resistance management
  • Screening

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