Use of the roche lightcycler strep B assay for detection of group B streptococcus from vaginal and rectal swabs

James R. Uhl, Emily A. Vetter, Kristi L. Boldt, Bruce W. Johnston, Kirk D. Ramin, Maria J. Adams, Patricia Ferrieri, Udo Reischl, Franklin R. Cockerill

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The results for a real-time PCR assay, using the LightCycler Strep B analyte-specific reagents (Roche Diagnostics Corporation, Indianapolis, Ind.), were compared to a direct plate method combined with a broth enrichment culture method for detection of group B streptococcus colonization in pregnant women. Two separate evaluations were conducted using two different automated nucleic extraction instruments, the MagNA Pure LC instrument (Roche Diagnostics Corporation) and the lower-capacity MagNA Pure Compact instrument (Roche Diagnostics Corporation). The sensitivities, specificities, and positive and negative predictive values for the different evaluation methods were as follow: for the LightCycler Strep B assay with MagNA Pure LC, 100, 97, 90, and 100%, respectively; for the LightCycler Strep B assay with MagNA Pure Compact, 92.5, 99, 97, and 97.5%, respectively. The LightCycler Strep B assay combined with either MagNA Pure LC or MagNA Pure Compact extraction is a suitable method for detecting group B streptococcus colonization in pregnant women. An advantage of the LightCycler assay over culture is the considerably reduced turnaround time for results.

Original languageEnglish (US)
Pages (from-to)4046-4051
Number of pages6
JournalJournal of clinical microbiology
Volume43
Issue number8
DOIs
StatePublished - Aug 2005

Fingerprint Dive into the research topics of 'Use of the roche lightcycler strep B assay for detection of group B streptococcus from vaginal and rectal swabs'. Together they form a unique fingerprint.

Cite this